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Denaturation Solution
基本信息
| NACRES | NA.25 |
| General description【一般描述】 | Sigma′s denaturing solution is a strong base that denatures double-stranded DNA so that it can be efficiently transferred to nylon membrane and subsequently hybridized to labeled probe. When used with a depurination solution, treatment results in the hydrolysis of the phosphodiester backbone at the sites of depurination, allowing for more efficient transfer of large DNA in neutral Southern transfers. |
| Application【应用】 | Suitable for denaturing dsDNA before transfer to membranes during Southern procedures. |
| Components【组分】 | The denaturing solutions is composed of 0.5N NaOH, and 1.5M NaCl. |
| Other Notes【其他说明】 | 0.5 N NaOH, 1.5 M NaCl |
产品性质
| Quality Level【质量水平】 | 200 |
| grade【等级】 | for molecular biology |
| form【形式】 | solution |
| storage temp.【储存温度】 | room temp |
安全信息
| Pictograms【象形图】 | GHS05 |
| Signal word【警示用语：】 | Danger |
| Hazard Statements | H290 - H314 |
| Precautionary Statements | P234 - P280 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338 - P363 |
| Hazard Classifications【危险分类】 | Eye Dam. 1 - Met. Corr. 1 - Skin Corr. 1B |
| Storage Class Code【储存分类代码】 | 8B - Non-combustible, corrosive hazardous materials |
| WGK | WGK 1 |
