广州伟伯科技有限公司
您的位置:首页 > 产品中心 > DLD1 CELLS AKT2 -/-
产品搜索:

DLD1 CELLS AKT2 -/-

产品编号:3079207
包装规格:1 SET
产品类别:进口试剂
品牌:Sigma-Aldrich
优惠价:立即咨询
产品价格
产品编号包装单位单价(元)国内现货国外库存询价单
30792071 SET59050
基本信息
NACRES
NA.81
General description【一般描述】
DLD1 Cells SMAD4 -/- are colorectal adenocarinoma, epithelial cells, from a human adult male, with a ZFN knock out modification.
This product corresponds to ATCC Cat. No. CCL-221.
Application【应用】
Media Renenewal changes two to three times per week.
Rapidly thaw vial by gentle agitation in 37℃ water bath (~2 minutes), keeping vial cap out of the water. Decontaminate with 70% ethanol, add 9 mL culture media and centrifuge 125 x g (5-7 minutes). Resuspend in complete culture media and incubate at 37℃ in a 5% CO2 atmosphere.
Subculture ratio: approx. 1:3 to 1:10.
The base medium for this cell line is RPMI, Cat. No. R5886. To make the complete growth medium, add the following components to the base medium: fetal bovine serum, Cat. No. F4135, to a final concentration (v/v) of 10%, L-glutamine, Cat. No. G7513, to a final concentration of 2 mM and sodium pyruvate, Cat. No. S8636, to a final concentration of 1 mM.
Cell freezing medium-DMSO 1X, Cat. No. C6164.
Features and Benefits【特点和优势】
These DLD-1 cells are adherent, with a doubling time of approx. 20 hours.
Zinc finger nuclease (ZFN) knock out on chromosome 19q13.1-q13.2
Quality【质量】
Tested for Mycoplasma, sterility, post-freeze viability, short terminal repeat (STR) analysis for cell line identification, PCR assay for cell line species confirmation.
Components【组分】
组份可单独销售
DLD1 Control Cells
DLD1 CELLS AKT2 -/-
产品性质
biological source【生物来源】
human male colon (Source Disease: Colorectal adenocarcinoma)
Quality Level【质量水平】
200
OMIM accession no.【OMIM登记号】
164731
storage temp.【储存温度】
−196℃
Gene Information
human ... AKT2(208)
安全信息
Storage Class Code【储存分类代码】
10 - Combustible liquids
WGK
WGK 3
广州伟伯科技有限公司 版权所有 CopyRight ©2006-2024, All Rights Reserved
工信部备案号:粤ICP备08114744号   Page Run Time: 0.0125