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Duolink® In Situ Microplate Nuclear Stain, Anti-Fade
基本信息
| NACRES | NA.32 |
| Application【应用】 | Duolink®proximity ligation assay(PLA®) allows for endogenous detection of protein interactions, post translational modifications, and protein expression levels at the single molecule level in fixed cells and tissue samples. Use the Multiwell Plates modifications to the Duolink® In Situ Fluorescence Protocol to run an experiment with this product. A set of short instructions can also be used. Visit our Duolink® PLA Resource Center for information on how to run a Duolink® experiment, applications, troubleshooting, and more. To perform a complete Duolink® PLA in situ experiment you will need two primary antibodies (PLA, IHC, ICC or IF validated) that recognize two target epitopes. Other necessary reagents include a pair of PLA probes from different species (one PLUS and one MINUS), detection reagents, wash buffers, and mounting medium. Note that the primary antibodies must come from the same species as the Duolink® PLA probes. Analysis is carried out using standard immunofluorescence assay equipment. Specificity Duolink® In Situ Microplate Nuclear Stain and Anti-Fade are intended to be used after staining cells with Duolink® In Situ in microtiter plates. See the datasheet for more information. Application Note Two primary antibodies raised in different species are needed. Test your primary antibodies (IgG-class, mono- or polyclonal) in a standard immunofluorescence (IF), immunohistochemistry (IHC) or immunocytochemistry (ICC) assay to determine the optimal fixation, blocking, and titer conditions. Duolink® in situ reagents are suitable for use on fixed cells, cytospin cells, cells grown on slide, formalin-fixed, paraffin embedded (FFPE), or tissue (fresh or frozen). No minimum number of cells is required. Let us do the work for you, learn more about our Custom Service Program to accelerate your Duolink® projects View full Duolink® product list |
| Features and Benefits【特点和优势】 |
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| Legal Information【法律信息】 | Duolink is a registered trademark of sigma-aldrich Co. LLC PLA is a registered trademark of sigma-aldrich Co. LLC |
产品性质
| Quality Level【质量水平】 | 200 |
| product line【产品线】 | Duolink® |
| technique(s) | proximity ligation assay: suitable |
| fluorescence【荧光】 | λex 360 nm; λem 460 nm |
| suitability【适用性】 | suitable for fluorescence-detection automated sequencing suitable for microtiter plates |
| shipped in【运输】 | dry ice |
| storage temp.【储存温度】 | −20℃ |
安全信息
| Pictograms【象形图】 | GHS07,GHS09 |
| Signal word【警示用语：】 | Warning |
| Hazard Statements | H317 - H411 |
| Precautionary Statements | P261 - P272 - P273 - P280 - P302 + P352 - P333 + P313 |
| Hazard Classifications【危险分类】 | Aquatic Chronic 2 - Skin Sens. 1 |
| Storage Class Code【储存分类代码】 | 10 - Combustible liquids |
| WGK | WGK 3 |
