Duolink^® In Situ Orange Starter Kit Mouse/Goat

100

Duolink^®

proximity ligation assay: suitable

λ_ex 554 nm; λ_em 576 nm (orange) (Cyanine 3; Zeiss Filter set 20)

suitable for fluorescence

Duolink^®proximity ligation assay(PLA^®) allows for endogenous detection of protein interactions, post translational modifications, and protein expression levels at the single molecule level in fixed cells and tissue samples.

Follow the Duolink^® In Situ Fluorescence Protocol to use this product. A set of short instructionsis also available.

Visit our Duolink^® PLA Resource Center for information on how to run a Duolink^® experiment, applications, troubleshooting, and more.

To perform a complete Duolink^® PLA in situ experiment you will need two primary antibodies (PLA, IHC, ICC or IF validated) that recognize two target epitopes. This starter kit supplies all other necessary reagents for 30 Duolink^® PLA reactions, which include a pair of PLA probes (Anti-Mouse PLUS and Anti-Goat MINUS), orange detection reagents, wash buffers, and mounting medium.Note that the primary antibodies must come from the same species as the Duolink^® PLA probes. Analysis is carried out using standard immunofluorescence assay equipment.

Specificity
The Duolink^® In Situ Orange Starter Kit Mouse/Goat requires one primary antibody from mouse and one primary antibody from goat. Orange fluorescence detection reagents are often used with Cyanine 3 filter.

Application Note
Two primary antibodies raised in different species are needed. Test your primary antibodies (IgG-class, mono- or polyclonal) in a standard immunofluorescence (IF), immunohistochemistry (IHC) or immunocytochemistry (ICC) assay to determine the optimal fixation, blocking, and titer conditions. Duolink^® PLA in situ reagents are suitable for use on fixed cells, cytospin cells, cells grown on slide, formalin-fixed, paraffin embedded (FFPE), or tissue (fresh or frozen). No minimum number of cells is required.

Let us do the work for you, learn more about our Custom Service Program to accelerate your Duolink^® projects

View full Duolink^® product list

Duolink is a registered trademark of sigma-aldrich Co. LLC
PLA is a registered trademark of sigma-aldrich Co. LLC

组份可单独销售
DUO92006 Duolink^® In Situ PLA^® Probe Anti-Goat MINUS, Affinity purified Donkey anti-Goat IgG (H+L)
DUO92001 Duolink^® In Situ PLA^® Probe Anti-Mouse PLUS
DUO92007 Duolink^® In Situ Detection Reagents Orange
DUO82049 Duolink^® In Situ Wash Buffers, Fluorescence
DUO82040 Duolink^® In Situ Mounting Medium with DAPI

GHS05,GHS07,GHS09

Warning

H290 - H317 - H411

P234 - P261 - P272 - P273 - P280 - P302 + P352

Aquatic Chronic 2 - Met. Corr. 1 - Skin Sens. 1

8A - Combustible, corrosive hazardous materials

WGK 3