肠激酶 来源于牛肠

3.4.21.9 ( BRENDA | IUBMB )

232-761-1

MFCD00131020

32160410

NA.56

Enterokinase also referred to as enteropeptidase, is a transmembrane protein. This intestinal protease contains a heavy chain and a light chain linked by a disulfide bond. The amino-terminal sequence of the bovine enteropeptidase is homologous to trypsin-like serine proteases. Enterokinase is a highly specific serine protease that is used for the removal of the FLAG peptide from N-terminal and Met-N-terminal fusion proteins. It does not remove the C-terminal FLAG.

Enterokinase from bovine intestine has been used to remove S-Tag and N-terminal His-tag from recombinant glutathione peroxidase 1 (Gpx1). It has also been used to cleave interleukin-2 (IL-2) protein from the granules
肠激酶是 S1 肽酶家族的一员。在体内，它负责胰蛋白酶原胰蛋白酶的蛋白水解活化。肠激酶用于位点含有可进入肠激酶识别位点的重组融合蛋白进行位点特异性切割，以去除亲和标签。

Enterokinase catalyzes the proteolytic activation of pancreatic proteases. This action prevents the harmful tissue damage produced by the autoactivation of pancreatic proteases in the pancreas. Enterokinase recognizes Lys or Arg residues in the peptide. Lack of enterokinase can harm food digestion and absorption mechanism. Enterokinase is a highly specific serine protease that is used for the removal of the FLAG peptide from N-terminal and Met-N-terminal fusion proteins. It does not remove the C-terminal FLAG.

One unit is that amount of enterokinase which results in >95% cleavage of 1 mg of purified FLAG-BAP fusion protein in 18 hours at 37 ℃. One FLAG-BAP unit is equal to 10x the activity of a standard trypsinogen unit.

不得使用 PVDF，因为游离标记肽将与 PVDF 膜结合。

powder

200

150 kDa (consisting of 115kDa and 35kDa subunits.)

white

dry ice

−20℃

与优化的肠激酶缓冲液共同提供

11 - Combustible Solids

WGK 3

Eyeshields, Gloves, type N95 (US)