表皮生长因子 来源于小鼠

NA.77

表皮生长因子(EGF)是小的促有丝分裂多肽(∼6 kDa)，存在于许多哺乳动物物种中，分布在许多组织和体液中。四种ErbB (HER) 家族受体酪氨酸激酶(包括EGFR/ErbB1、ErbB2、 ErbB3和 ErbB4)介导针对EGF家族成员的反应。人类和小鼠的EGF非常相似，但是它们的物理和化学性质并不相同。在构成两种多肽中每一种多肽的53个氨基酸残基中，有37个是两种分子共有的，并且在相同的相对位置上形成三个二硫键。

来自小鼠的表皮生长因子已用作基础(N2B27)培养基补充剂，用于培养分化的小鼠胚胎干(E14)细胞。

EGF(表皮生长因子)可参与细胞增殖、分化和存活。 此外，它还可影响各种生物活动，如血管生成、抑制胃酸分泌、调节多种激素的合成、细胞外基质蛋白质的合成和转换、骨组织钙释放(从而促进骨吸收)、成纤维细胞和上皮细胞的化学趋化作用、单独或与其他细胞因子组合介导伤口愈合过程。 EGF对于多种细胞类型具有促有丝分裂作用，包括成纤维细胞、上皮细胞、内皮细胞、软骨细胞、以及SV40-3T3细胞。

该产品从磷酸盐缓冲盐水(pH 7.4，0.2 μm滤膜过滤)中冻干。

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200

mouse

expressed in E. coli

≥90% (SDS-PAGE)

lyophilized powder

0.05-1 ng/mL ED₅₀/EC₅₀

~6 kDa

pkg of 100 μg

avoid repeated freeze/thaw cycles

cell culture | mammalian: suitable

≤1 EU/μg endotoxin (Protein)

white

water: soluble 0.10 mL, clear, colorless

P01132

−20℃

mouse ... Egf(13645)

13 - Non Combustible Solids

WGK 1