Fpg Protein from Escherichia coli

3.2.2.23 ( BRENDA | IUBMB )

MFCD02264758

NA.32

Fpg contains two domains separated by a flexible hinge.
Formamidopyrimidine-DNA glycosylase (Fpg) is a DNA repair enzyme found in Escherichia coli, which contains one zinc atom. Proximal to its C-terminal, it contains a zinc-finger motif of CC/CC type.

Fpg Protein from Escherichia coli has been used for the assessment of DNA oxidative damage using comet assay.

Fpg is a key enzyme in the DNA base excision repair pathway (BER). It catalyses the excision of a broad spectrum of modified purines. Fpg has both DNA glycosylase activity that removes the mutated base and AP-lyase activity that releases ribose leaving both 5′- and 3′-phosphorylated ends in the DNA. The zinc finger motif at its C-terminus is responsible for the DNA binding and AP-lyase activity. In addition, its N-terminal proline acts as a nucleophile to produce a Schiff base intermediate that is essential for enzyme action.
Fpg specifically acts on 3′- and 5′-phosphodiester bonds.
Formamidopyrimidine-DNA glycosylase (Fpg) cleaves double-stranded DNA containing the damaged base 8-oxo-7,8-dihydroguanine. It functions as an N-glycosylase and apurinic/apyrimidinic lyase.

One unit will cleave 50% of 0.5 pmol of double-stranded DNA oligomer substrate (8-oxoguanine−mutated) in 10 min at 25 ℃.

Solution in 50% glycerol containing 50 mM potassium HEPES, pH 7.5, 1 mM DTT, 1 mM EDTA, and 200 mM NaCl.

200

expressed in E. coli

≥90% (SDS-PAGE)

buffered aqueous glycerol solution

>20,000 units/mg protein

30.2 kDa (269 amino acids, predicted from the nucleotide sequence)

P05523

genomic analysis

wet ice

−20℃

Escherichia coli CFT073 ... mutM(1038243)
Escherichia coli K12 ... mutM(946765)

10 μg in poly bottle

10 - Combustible liquids

WGK 2

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)