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Glycerol 3-phosphate Oxidase from Pediococcus sp.
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| 产品编号 | 包装单位 | 单价(元) | 国内现货 | 国外库存 | 询价单 |
| 3091502 | 100 UNITS | 1740 |
产品别名
9046-28-0
Glycerol 3-phosphate Oxidase from Pediococcus sp.
sn-Glycerol 3-phosphate:oxygen 2-oxidoreductase
基本信息
| Enzyme Commission (EC) Number【酶学委员会(EC)编号】 | 1.1.3.21 ( BRENDA | IUBMB ) |
| EC number【EC 号】 | 232-932-0 |
| MDL number | MFCD00131209 |
| NACRES | NA.54 |
| General description【一般描述】 | Glycerol 3-phosphate Oxidase is a FAD-dependent α-glycerophosphate oxidase and catalyzes the formation of dihydroxyacetonephosphate. Glycerol 3-phosphate oxidase comprises of a flavin adenine dinucleotide (FAD) and a substrate binding domain and corresponds to molecular weight of 42 kDa. It exists as a monomer and the active site comprises of isoalloxazine ring and histidine 53, arginine 316 and lysine 345 residues crucial for ligand binding. |
| Application【应用】 | Glycerol 3-phosphate Oxidase from Pediococcus sp. has been used in the kinetic assay to study gluconeogenesis in liver and in immobilization studies on pencil graphite (PG) electrode. This enzyme is useful for enzymatic determination of triglyceride when coupled with lipoprotein lipase (LPL-311, LPL-314) and glycerokinase (GYK-301, GYK-311) in clinical analysis. |
| Physical properties【物理属性】 | Isoelectric point : 4.1 -/+0.1 Michaelis constants : 3.2x10 -3M (L-α-Glycerophosphate), 6.8 x 10 -3M (D, L-form) Inhibitors : Ionic detergents (SDS, LBS, etc.), Hg++, Ag+ Optimum pH : 8.0 - 8.5Optimum temperature : 35 - 40oC pH Stability : pH 6.5 - 8.5 (25oC, 20hr) Thermal stability : below 40oC (pH 7.0, 15min) Substrate specificity : The enzyme has the highest specificity for L-form of a-γlycerophosphate |
| Unit Definition【单位定义】 | One unit will oxidize 1.0 μmole of |
| Physical form【外形】 | Lyophilized powder containing stabilizers |
产品性质
| form【形式】 | lyophilized powder |
| Quality Level【质量水平】 | 200 |
| Specific Activity | 40-80 units/mg solid, pH 8.1 |
| mol wt【分子量】 | ~76 kDa (gel filtration) |
| storage temp.【储存温度】 | 2-8℃ |
| packaging【包装】 | 100 units in glass bottle |
安全信息
| Storage Class Code【储存分类代码】 | 13 - Non Combustible Solids |
| WGK | WGK 3 |
| Personal Protective Equipment【个人防护装备】 | Eyeshields, Gloves, type N95 (US) |
