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产品性质
| biological source【生物来源】 | human bone |
| growth mode【生长模式】 | Adherent |
| karyotype【核型】 | 2n = 46, hyperdiploid |
| morphology【形态学】 | Epithelial-like |
| products【产品】 | Not specified |
| receptors【受体】 | Not specified |
| technique(s) | cell culture | mammalian: suitable |
| relevant disease(s)【相关疾病】 | cancer |
| shipped in【运输】 | dry ice |
| storage temp.【储存温度】 | −196℃ |
基本信息
| Cell Line Origin【细胞系来源】 | Human Caucasian osteosarcoma, TE85 |
| Cell Line Description【细胞系描述】 | Derived from a 13 year old female caucasian. Cells are sensitive to both virus and chemical transformation and exhibit a flat morphology, low saturation density and low plating efficiency in soft agar. HOS and 143B (Sigma Catalogue number 91112502) originated from the same host: 143B is TK- and originated indirectly from HOS which is TK+ (Skovgaard et al 2010 PMID: 20544518). |
| Application【应用】 | Virus, transformation and biochemical studies |
| DNA Profile【DNA图谱分析】 | STR-PCR Data: Amelogenin: X CSF1PO: 12 D13S317: 12 D16S539: 10,13 D5S818: 13 D7S820: 11,12 THO1: 6 TPOX: 8,11 vWA: 18 |
| Culture Medium【培养基】 | EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS). |
| Subculture Routine【传代培养常规】 | Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37℃. |
| Other Notes【其他说明】 | Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information. |
