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HT 1080

产品编号:3097363
规格:85111505
包装规格:20μl,5μg
产品类别:进口试剂
优惠价:立即咨询
产品价格
产品编号包装单位单价(元)国内现货国外库存询价单
309736320μl4470
30973635μg4630
30973635μg5400
产品性质
biological source【生物来源】
human fibrosarcoma
growth mode【生长模式】
Adherent
karyotype【核型】
2n = 46, pseudodiploid
morphology【形态学】
Epithelial
products【产品】
Not specified
receptors【受体】
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)【相关疾病】
cancer
shipped in【运输】
dry ice
storage temp.【储存温度】
−196℃
基本信息
Cell Line Origin【细胞系来源】
Human fibrosarcoma
Cell Line Description【细胞系描述】
Established from a fibrosarcoma arising adjacent to the acetabulum of a 35 year old caucasian male. The cells can give rise to tumours in immunosupressed NIH Swiss mice. Susceptible to infection by RNA tumour viruses including RD114 feline endogenous virus and FeLV.
Application【应用】
HT 1080 has been used to demonstrate that type I collagen induces matrix metallopeptidase 2 (MMP-2) activation. It has also been used to study the roles of Ras-related C3 botulinum toxin substrate 1 and 2 (Rac1 and Rac2) in regulating morphology, F-actin organization, adhesion, migration and chemotaxis of HT1080 cells.
DNA Profile【DNA图谱分析】
STR-PCR Data: Amelogenin: X,Y,
CSF1PO: 12
D13S317: 12,14
D16S539: 9,12
D5S818: 11,13
D7S820: 9,10
THO1: 6
TPOX: 8
vWA: 14,19
Culture Medium【培养基】
EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).
Subculture Routine【传代培养常规】
Split sub-confluent cultures (70-80%) 1:4 to 1:8 i.e. seeding at 1-4x10,000 cells/cm2 using 0.25% trypsin/EDTA; 5% CO2; 37℃.
Other Notes【其他说明】
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
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