IgG1-APC Isotype Control murine

NA.42

This mouse IgG1 kappa monoclonal antibody (clone MOPC-21) has unknown specificity and was chosen as an isotype control after screening on variety of resting, activated, live and fixed rat and human tissues.
The specificity of staining by monoclonal antibodies to target antigens should be verified by establishing the amount of non-specific antibody binding. Especially at higher concentration (more than 15 μg/ml) the antibody staining usually has consignable background. To this end a non-reactive immunoglobulin of the same isotype is included as a negative control for each specific monoclonal antibody used in a particular immunoassay. The monoclonal antibody MOPC-21, generated against an undefined antigen, does not react specifically with rat and human samples, and hence all the background that could be observed when working with this antibody would be a result of general nonspecific interactions between an mouse IgG1 molecule and the respective sample under the particular conditions.

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Solution in phosphate buffered saline containing 15 mM sodium azide and 0.2% high-grade protease free BSA as a stabilizing agent.

100

mouse

Allophycocyanin conjugate

purified immunoglobulin

MOPC-21, monoclonal

buffered aqueous solution

0.1 mg/mL

flow cytometry: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable

IgG1

wet ice

2-8℃

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12 - Non Combustible Liquids

WGK 2