JumpStart ^TM AccuTaq ^TM LA DNA 聚合酶

NA.55

JumpStart^™ AccuTaq^™ LA DNA Polymerase is a combination of Sigma′s high-fidelity blend, AccuTaq long and accurate (LA) DNA Polymerase, and the Taq neutralizing JumpStart Taq antibody. This specially formulated enzyme is the best choice for long-distance and high-fidelity PCR, multiplex PCR, and PCR amplification of targets with variable lengths, such as amplification of cDNA libraries. JumpStart Taq antibody reversibly binds to the AccuTaq LA DNA Polymerase, inactivating it at room temperature. The increased temperature of the first denaturation cycle causes the complex to dissociate, restoring the enzyme activity. This hot-start mechanism provides increased specificity and higher target yield in comparison to standard amplification.

JumpStart^™ AccuTaq^™ LA DNA Polymerase has been used:

• in polymerase chain reaction (PCR) for the amplification of soil DNA samples
• in accurate long range PCR of histone H2A.Z in bacterial artificial chromosome (BACs).
• as a component of the PCR reaction mix for amplification of viral DNA through conventional PCR

在 74℃ 下一个单位在 30 min 内将 10 nmol 的总 dNTP 掺入到酸可沉淀的 DNA 中。

请在www.sigma-aldrich.com/hotstart页面查看有关 Accutaq 产品的更多详细信息。

本产品的使用受到如下一项或多项美国专利及其对应的境外专利声明保护：5,789,224, 5,618,711, 6,127,155以及与届满的美国专利号5,079,352对应的境外专利声明。购买本产品，即相当于依照境外的专利声明获得了一份受限制、不可转让的使用许可，即将此等数量的产品用于购买者内部的研究用途。我们未明确表示、暗示或以禁止反言的形式授予您任何其他专利声明下的权利、进行任何专利方法申请的权利、进行任何形式的商业服务的权利，包括但不限于出于收费或其他商业考虑而报告购买者的研究活动结果的权利。本产品仅适合于研究用途。如需用于Roche专利许可的诊断用途，需另外征得Roche许可。有关购买许可的更多信息，可联系Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA获取。
AccuTaq is a trademark of sigma-aldrich Co. LLC
Jacobs is a registered trademark of Jacobs
JumpStart is a trademark of sigma-aldrich Co. LLC

200

liquid

sufficient for 125 reactions
sufficient for 500 reactions

Long & Accurate PCR
dNTPs included: no
hotstart

2.5 unit/μL

PCR: suitable

colorless

purified DNA

wet ice

−20℃

提供 10× 反应缓冲液。

10 - Combustible liquids

WGK 3

Eyeshields, Gloves, type ABEK (EN14387) respirator filter