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产品性质
biological source【生物来源】 | human blood |
growth mode【生长模式】 | Suspension |
karyotype【核型】 | 46XY,+t(8,?13)(p12-21,?q13)+(8,?3)(p12-21,?q21)+I |
morphology【形态学】 | Lymphoblastoid |
products【产品】 | Not specified |
receptors【受体】 | Not specified |
technique(s) | cell culture | mammalian: suitable |
relevant disease(s)【相关疾病】 | cancer |
shipped in【运输】 | dry ice |
storage temp.【储存温度】 | −196℃ |
基本信息
Cell Line Origin【细胞系来源】 | Human Caucasian B-prolymphocytic leukaemia, EBV transformed |
Cell Line Description【细胞系描述】 | Derived from the peripheral blood of a human Caucasian male patient with prolymphocytic leukaemia. Cells have been subsequently transformed by treatment with EBV and TPA (12-0-tetradecanoylphorbol-13-acetate). JVM-13 cells express surface-membrane and cytoplasmic lambda light chain immunoglobulin. Immunological markers detected were HLA-DR, CD19, CD20, CD21, FMC7, CD38, CD71 and CD9. |
Application【应用】 | Study of B cell leukaemia |
DNA Profile【DNA图谱分析】 | STR-PCR Data: Amelogenin: X,Y CSF1PO: 10,12 D13S317: 9,11 D16S539: 11 D5S818: 11 D7S820: 9 THO1: 6,7 TPOX: 10,11 vWA: 16,18 |
Culture Medium【培养基】 | RPMI 1640 + 4mM Glutamine + 10% Foetal Bovine Serum (FBS). |
Subculture Routine【传代培养常规】 | Maintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37℃. |
Other Notes【其他说明】 | Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information. |