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产品性质
biological source【生物来源】 | human bone marrow |
growth mode【生长模式】 | Suspension |
karyotype【核型】 | 2n = 46, pseudodiploid |
morphology【形态学】 | Lymphoblast |
products【产品】 | Not specified |
receptors【受体】 | Not specified |
technique(s) | cell culture | mammalian: suitable |
relevant disease(s)【相关疾病】 | cancer |
shipped in【运输】 | dry ice |
storage temp.【储存温度】 | −196℃ |
基本信息
Cell Line Origin【细胞系来源】 | Human Caucasian bone marrow acute myelogenous leukaemia |
Cell Line Description【细胞系描述】 | Derived from KG-1, however KG1a is unresponsive to colony stimulating factor in soft agar culture and does not express the Ia-like antigen. KG1a is resistant to phorbol-diester induced macrophage differentiation and cell proliferation. KG1a cells are morphologically, cytochemically and functionally less mature than the parental KG-1. Expresses HLA A30,31; B35; CW4. |
DNA Profile【DNA图谱分析】 | STR-PCR Data: Amelogenin: X,Y CSF1PO: 7 D13S317: 11,12 D16S539: 10,11 D5S818: 13 D7S820: 8,10 THO1: 7,8 TPOX: 7,9 vWA: 14,19 |
Culture Medium【培养基】 | IDMEM + 2mM Glutamine + 20% Foetal Bovine Serum (FBS). |
Subculture Routine【传代培养常规】 | Maintain cultures between 5x100,000 to 1,000,000 cells/ml. Do not culture below 100,000 cells/ml; 5% CO2; 37℃. |
Other Notes【其他说明】 | Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information. |