L -谷氨酸氧化酶 来源于链霉菌 属

MFCD00131459

NA.54

This product has a 6xHistidine tag with a thrombin cleavage site.
L-Glutamate Oxidase (LGOX) from Streptomyces sp is secreted extracellularly in the wheat bran pre cultivation medium. It corresponds to a molecular weight of 120 kDa with monomer subunit of 61 kDa. The structure of LGOX comprises of hexamer and has flavin adenine dinucleotide prosthetic group.

L-Glutamate Oxidase from Streptomyces sp may be used:

• as a reference enzyme in hydrogelpoly(carbamoylsulphonate) based immobilization for biosensor development
• as a component of pencil graphite electrode for biosensing L-glutamate

L-Glutamate Oxidase (LGOX) catalyzes the oxidative deamination of L-glutamate to α-ketoglutarate, ammonia and hydrogen peroxide. LGOX is industrially produced and is exploited majorly as a biosensor for L-glutamate detection and in the L-glutamate kits.

One unit will form 1.0 micromole of alpha-ketoglutaric acid from L-glutamic acid per minute at pH 7.4 at 30 deg C.

200

expressed in E. coli

solid

≥5 units/mg protein

120 kDa

−20℃

13 - Non Combustible Solids

WGK 3