您的位置：首页 > 产品中心 > 9001-65-4， 甘露醇脱氢酶 来源于肠系膜明串珠菌
甘露醇脱氢酶 来源于肠系膜明串珠菌
Mannitol Dehydrogenase from Leuconostoc mesenteroides
产品别名
9001-65-4
甘露醇脱氢酶 来源于肠系膜明串珠菌
Mannitol Dehydrogenase from Leuconostoc mesenteroides
Mannitol:NAD oxidoreductase
polyol dehydrogenases
甘露醇:NAD 氧化还原酶
基本信息
| Enzyme Commission (EC) Number【酶学委员会(EC)编号】 | 1.1.1.67 ( BRENDA | IUBMB ) |
| MDL number | MFCD00131575 |
| NACRES | NA.54 |
| General description【一般描述】 | Mannitol dehydrogenase (MDH) is a NAD-dependent enzyme, obtained from several organisms like, Lactobacillus brevis, Leuconostoc mesenteroides, Absidia glauca and Rhodococcus. MDH obtained from L. mesenteroides exhibits similarity in sequence with the medium-chain dehydrogenase/reductase protein family. It also contains four identical subunits of about 38 kDa in size. The molecular weight of mannitol dehydrogenase is 136 kDa. Mannitol dehydrogenase is a cytoplasmic enzyme whose primary role is the regulation of intracellular levels of the sugar alcohol mannitol in plants. It catalyzes the pyridine nucleotide dependent reduction of fructose to mannitol. |
| Application【应用】 | It has been used in a study to assess glucosylglycerol and glucosylglycerate as enzyme stabilizers. Can be used for the production of D-mannitol. This preparation is useful in the determination of mannitol in urine. |
| Biochem/physiol Actions【生化/生理作用】 | Mannitol dehydrogenase (MDH) helps to catalyze the pyridine nucleotide-dependent oxidation of |
| Unit Definition【单位定义】 | 在 pH 5.3 和 30℃ 且存在 NADH 的条件下，一单位本品每分钟将还原 1.0μmol |
| Physical form【外形】 | 含缓冲盐、磷酸钾以及二硫苏糖醇的冻干粉 |
| Preparation Note【制备说明】 | 部分纯化 |
| Analysis Note【分析说明】 | 根据 Yamanaka, Meth. Enzymol., 41(B), 138 (1975) 所述方法进行分析。 |
产品性质
| form【形式】 | lyophilized powder |
| Quality Level【质量水平】 | 200 |
| Specific Activity | ≥60 units/mg protein |
| mol wt【分子量】 | 136 kDa |
| storage temp.【储存温度】 | −20℃ |
安全信息
| Storage Class Code【储存分类代码】 | 12 - Non Combustible Liquids |
| WGK | WGK 3 |
| Personal Protective Equipment【个人防护装备】 | Eyeshields, Gloves |
