甲基绿

C₂₇H₃₅BrClN₃ · ZnCl₂

653.24

42590

3914106

230-415-4

MFCD00151094

24897293

NA.47

Methyl green is a synthetic, triphenylmethane-type cationic dye related to ethyl green mainly used as DNA stain in histochemistry. It possesses aniline rings with different degrees of methylation. The dye binds specifically to the A-T rich regions of the major groove of DNA with the help of two positive charges. Methyl green in combination with pyronin is extensively used to stain RNA and plasma cells in tissue sections and cytologic preparations.

Methyl green is suitable as a nuclear counterstain in enzyme histochemistry, immunostaining, and in situ hybridization as the bluish-green color will be in contrast with red, purple, brown, and black products of histochemical reactions. It is also suitable as a tracking dye in Acid-polyacrylamide gel electrophoresis to separate gliadins. It has been used in the histochemical staining for the apoptosis examination of kidney tissue and as a nuclear stain for labeling embryos.

材料可以是一溴一氯和二氯 ZnCl₂ 盐的混合物。标签列出了卤素含量。

基于一溴一氯 ZnCl₂ 盐分子量 (653.2)的染料含量。

200

~85%

powder

Dye Content, ≥83%

0.2 - 1.8, yellow to blue

>300 ℃ (lit.)

water: 1 mg/mL

>11100.00 at 417-425 nm in water at 0.005 g/L
>16000 at 311-319 nm in water at 0.005 g/L
>60000 at 626-634 nm in water at 0.005 g/L

diagnostic assay manufacturing
hematology
histology

room temp

[Cl-].[Br-].Cl[Zn]Cl.CC[N+](C)(C)c1ccc(cc1)\C(c2ccc(cc2)N(C)C)=C3/C=C\C(C=C3)=[N+](\C)C

1S/C27H35N3.BrH.3ClH.Zn/c1-8-30(6,7)26-19-13-23(14-20-26)27(21-9-15-24(16-10-21)28(2)3)22-11-17-25(18-12-22)29(4)5;;;;;/h9-20H,8H2,1-7H3;4*1H;/q+2;;;;;+2/p-4

RRKSQWOQZFIQKW-UHFFFAOYSA-J

25 g in glass bottle
5 g in poly bottle

GHS05,GHS07,GHS09

Danger

H314 - H335 - H411

P260 - P271 - P273 - P280 - P303 + P361 + P353 - P305 + P351 + P338

Aquatic Chronic 2 - Eye Dam. 1 - Skin Corr. 1B - STOT SE 3

Respiratory system

8A - Combustible, corrosive hazardous materials

WGK 3

dust mask type N95 (US), Eyeshields, Gloves