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Monoclonal Anti- Cathepsin B 小鼠抗
Anti-Cathepsin B antibody, Mouse monoclonal
产品编号: | 3142175 |
规格: | clone CB59-4B11, purified from hybridoma cell culture |
包装规格: | 200 μL |
产品类别: | 进口试剂 |
品牌: | Sigma-Aldrich |
优惠价: | 立即咨询 |
产品别名
Monoclonal Anti- Cathepsin B 小鼠抗
Anti-Cathepsin B antibody, Mouse monoclonal
Anti-CYP1A2
Anti-CP12
Anti-P450(PA)
Anti-P3-450
产品性质
Quality Level【质量水平】 | 200 |
biological source【生物来源】 | mouse |
conjugate【偶联物】 | unconjugated |
antibody form【抗体形式】 | purified immunoglobulin |
antibody product type | primary antibodies |
clone【克隆】 | CB59-4B11, monoclonal |
form【形式】 | buffered aqueous solution |
mol wt【分子量】 | antigen ~25 kDa |
species reactivity | human, rat |
concentration【浓度】 | ~1.5 mg/mL |
technique(s) | immunohistochemistry: suitable indirect ELISA: suitable western blot |
isotype【同位素/亚型】 | IgG1 |
UniProt accession no.【UniProt登记号】 | P07858 |
shipped in【运输】 | dry ice |
storage temp.【储存温度】 | −20℃ |
Gene Information | human ... CTSB(1508) rat ... CTSB(64529) |
基本信息
General description【一般描述】 | The CTSB (Cathepsin B) gene encoding cathepsin B is mapped to human chromosome 8p23.1. Cathepsin B are normally localized in lysosomes of almost all mammalian cells, but under certain conditions they can be secreted from the cell that take part in local proteolysis. Cathepsin B is both an endo- and exopeptidase, composed of a dimer of disulfide-linked heavy and light chains, both produced from a single protein precursor. Monoclonal Anti-Cathepsin B specifically recognizes human1 and rat1 cathepsin B (~ 25 kDa). The antibody epitope resides within the peptide sequence EPGYSP which corresponds to amino acids 212-217 of human cathepsin B. The antibody does not cross react with the closely related lysosomal cysteine proteases: cathepsins L, H, K, S, V, and W |
Immunogen【免疫原】 | Recombinant human cathepsin B. |
Application【应用】 | indirect ELISA, immunohistochemistry, In HCT-116 cells. 4-8μg/mL of antibody concentration may be used. A dilution of 1:800 in human pancreatic carcinoma cell line PANC-1 was used for immunoblotting |
Biochem/physiol Actions【生化/生理作用】 | Cathepsins are lysosomal proteases activation of enzyme precursors, and tumor invasion and metastasis. It is involved in the proteolysis of many proteins involved in different physiological and pathological pathways. In the brain, this protease, also known as amyloid precursor protein secretase, is responsible for the proteolytic processing of amyloid precursor protein (APP). An incomplete proteolytic processing of APP has been found as a causative factor in Alzheimer disease. Furthermore, ablated genes coding cathepsin B and L leads to enormous neuronal cell death,probably due to autophagy abnormalities. Cathepsin B has been implicated in tumor progression by virtue of localization at the invading front of the tumor. For example, blocking cathepsin B expression in human glioblastoma SNB19 cells by a stable transfection of an expression vector expressing antisense cathepsin B, caused suppression of glioblastoma-induced neovascularization. Monoclonal Anti-Cathepsin B specifically recognizes human1 and rat1 cathepsin B (~ 25 kDa). The antibody epitope resides within the peptide sequence EPGYSP which corresponds to amino acids 212-217 of human cathepsin B. The antibody does not cross react with the closely related lysosomal cysteine proteases: cathepsins L, H, K, S, V, and W. Cathepsins significantly takes part in the immune response, by processing antigens into peptides for its presentation through MHC class II molecules. |
Physical form【外形】 | 0.01M 磷酸缓冲盐溶液,pH 7.4,含 15mM 叠氮化钠。 |
产品说明
Disclaimer【免责声明】 | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
安全信息
Storage Class Code【储存分类代码】 | 12 - Non Combustible Liquids |
WGK | WGK 3 |