Monoclonal Anti-CD20-Biotin antibody produced in mouse

NA.41

CD20 is a cell surface 33-37 (depending on the degree of phosphorylation) kDa non-glycosylated surface phosphoprotein expressed on mature and most malignant B cells, but not stem cells or plasma cells (low number of the CD20 has been also detected on a subpopulation of T lymphocytes and it can be expressed on follicular dendritic cells). Its expression on B cells is synchronous with the expression of surface IgM. CD20 regulates transmembrane calcium conductance (probably functioning as a component of store-operated calcium channel), cell cycle progression and B-cell proliferation. It is associated with lipid rafts, but the intesity of this association depends on extracellular triggering, employing CD20 conformational change and/or BCR (B cell antigen receptor) aggregation. After the receptor ligation, BCR amd CD20 colocalize and then rapidly dissociate before BCR endocytosis, whereas CD20 remains at the cell surface. CD20 serves as a useful target for antibody-mediated therapeutic depletion of B cells, as it is expressed at high levels on most B-cell malignancies, but does not become internalized or shed from the plasma membrane following mAb treatment.

The antibody 2H7 reacts with CD20 (Bp35), a 33-37 kDa non-glycosylated membrane receptor with four transmembrane domains, expressed on B lymphocytes (it is lost on plasma cells), follicular dendritic cells, and at low levels on peripheral blood T lymphocytes.

Human tonsillar B cells

The reagent is designed for Flow Cytometry analysis. Working concentrations should be determined by the investigator.

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Solution in phosphate buffered saline, pH 7.4, with 15 mM sodium azide.

100

mouse

biotin conjugate

purified immunoglobulin

primary antibodies

2H7, monoclonal

buffered aqueous solution

human, nonhuman primates

1 mg/mL

flow cytometry: suitable

IgG2a

NM_021950.3

P11836

wet ice

2-8℃

human ... MS4A1(931)

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