您的位置：首页 > 产品中心 > Monoclonal Anti-DFFA, (C-terminal) antibody produced in mouse
Monoclonal Anti-DFFA, (C-terminal) antibody produced in mouse
| 产品编号： | 3144067 |
| 规格： | clone 3A11, purified immunoglobulin, buffered aqueous solution |
| 包装规格： | 100 μG |
| 产品类别： | 进口试剂 |
| 品牌： | Sigma-Aldrich |
| 优惠价： | 立即咨询 |
产品别名
Monoclonal Anti-DFFA, (C-terminal) antibody produced in mouse
ICAD
DFF-45
DFF1
基本信息
| NACRES | NA.41 |
| General description【一般描述】 | Apoptosis is a cell death process that removes toxic and/or useless cells during mammalian development. The apoptotic process is accompanied by shrinkage and fragmentation of the cells and nuclei and degradation of the chromosomal DNA into nucleosomal units. DNA fragmentation factor (DFF) is a heterodimeric protein of 40-kD (DFFB) and 45-kD (DFFA) subunits. DFFA is the substrate for caspase-3 and triggers DNA fragmentation during apoptosis. DFF becomes activated when DFFA is cleaved by caspase-3. The cleaved fragments of DFFA dissociate from DFFB, the active component of DFF. DFFB has been found to trigger both DNA fragmentation and chromatin condensation during apoptosis. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. (provided by RefSeq) |
| Immunogen【免疫原】 | DFFA (NP_004392.1, 231 a.a. ~ 331 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence TSSDVALASHILTALREKQAPELSLSSQDLELVTKEDPKALAVALNWDIKKTETVQEACERELALRLQQTQSLHSLRSISASKASPPGDLQNPKRARQDPT |
| Biochem/physiol Actions【生化/生理作用】 | DNA fragmentation factor subunit α (DFFA) is a component of DNA fragmentation factor (DFF). It is mainly associated with apoptosis and genomic stability. Caspase-3-mediated cleavage of DFFA releases DFF40 (DNA fragmentation factor 40) which degrades chromosomal DNA. During menstruation, the apoptosis of endometrium cells is associated with DFFA. Its expression decreases after menopause. Silencing of DFFA increases doxorubicin effects in breast cancer cells. |
| Physical form【外形】 | Solution in phosphate buffered saline, pH 7.4 |
产品性质
| Quality Level【质量水平】 | 100 |
| biological source【生物来源】 | mouse |
| conjugate【偶联物】 | unconjugated |
| antibody form【抗体形式】 | purified immunoglobulin |
| antibody product type | primary antibodies |
| clone【克隆】 | 3A11, monoclonal |
| form【形式】 | buffered aqueous solution |
| mol wt【分子量】 | antigen ~37.22 kDa |
| species reactivity | human |
| technique(s) | indirect ELISA: suitable proximity ligation assay: suitable western blot: 1-5 μg/mL |
| isotype【同位素/亚型】 | IgG2aκ |
| NCBI accession no.【NCBI登记号】 | NM_004401 |
| UniProt accession no.【UniProt登记号】 | O00273 |
| shipped in【运输】 | dry ice |
| storage temp.【储存温度】 | −20℃ |
| Gene Information | human ... DFFA(1676) |
安全信息
| Storage Class Code【储存分类代码】 | 13 - Non Combustible Solids |
| WGK | WGK 1 |
