Monoclonal Anti-Dihydropyridine Receptor (α₁ Subunit) antibody produced in mouse

MFCD01633297

NA.41

DHP receptor is composed of at least four subunits. The α1 subunit contains the binding site for the DHPs and shows high sequence homology to the voltage gated Na+ channel. The α2 subunit is a large glycoprotein and the other 2 subunits are termed β and γ.

Reacts specifically with the α₁ subunit of the DHP receptor. May be used to inhibit the DHP-sensitive calcium current in BC3H1 mouse muscle cells. By immunohistochemistry, double rows of discrete punctate staining represent pairs of triads on the opposing sides of the Z-lines.

rabbit muscle transverse tubule dihydropyridine (DHP) receptors.

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunofluorescence (1 paper)
Monoclonal Anti-Dihydropyridine Receptor (α1 Subunit) antibody produced in mouse is suitable for immunoprecipitation, immunohistochemistry at a working dilution of 1:500 and immunoblotting at 1:2000. It was used for the detection and localization of DHPR-1α by western blotting in skeletal muscle tissue sections of mice in a study. The antibody may be used to inhibit the DHP-sensitive calcium current in BC3H1 mouse muscle cells.

The 1,4-dihydropyridines (DHPs) are synthetic organic compounds which can be used to identify the L-type Ca²⁺ channels that are found in all types of vertebrate muscle, neuronal and neuroendocrine cells. DHP receptor is part of the L-type calcium channel complex and is thought to be the voltage sensor in E-C coupling.

Solution in phosphate buffered saline containing 0.05% sodium azide.

200

unconjugated

ascites fluid

primary antibodies

1A, monoclonal

buffered aqueous solution

antigen ~200 kDa

guinea pig, rat, mouse, human (weakly), rabbit

immunohistochemistry (frozen sections): 1:200
immunoprecipitation (IP): suitable
western blot (chemiluminescent): 1:500

IgG1

Q13698

dry ice

−20℃

human ... CACNA1S(779)
mouse ... Cacna1s(12292)
rat ... Cacna1s(116652)

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