Anti-Glutathione-S-Transferase (GST) antibody, Mouse monoclonal

MFCD02253002

NA.41

Anti-Glutathione-S-transferase (GST) antibody, mouse monoclonal, (mouse IgG1 isotype) is derived from the hybridoma 2H3-D10 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a GST-fusion protein. GST is a Phase II metabolic enzyme, expressed ubiquitously in both eukaryotes and prokaryotes. GSTs are classified in to three types based on their cellular localization, namely cytosolic GSTs, mitochondrial GSTs and microsomal GSTs.

Monoclonal Anti- Glutathione-S-transferase (GST) recognizes GST from Schistosoma japonicum in GSTfusion proteins.

hybridoma 2H3-D10 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a GST-fusion protein

Anti-Glutathione-S-transferase (GST) antibody, Mouse monoclonal has been used in immunoblotting.

Glutathione-S-Transferase (GST) is an enzyme, which catalyzes the reaction of glutathione with a wide range of electrophilic substrates and therefore, plays a role in the detoxification of potential alkylating agents.(5) GST protects the cell against oxidative stress and several toxic molecules. It facilitates the synthesis and modification of leukotrienes and prostaglandins. GST from Schistosoma japonicum, has been cloned in several expression vectors as a 27.5 kDa molecule and used as a tag for expressing proteins of interest. These proteins are thus expressed as GST-fusion proteins. Antibodies to GST are useful in identifying the successful expression of these fusion proteins.

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide

mouse

unconjugated

purified immunoglobulin

primary antibodies

2H3-D10, monoclonal

buffered aqueous solution

~1.0 mg/mL

western blot: 0.2-0.4 μg/mL using detection limit for GST-fustion protein is ∼ 10ng/lane

dry ice

−20℃

Store at −20 ℃. For continuous use, store at 2-8 ℃ for up to one month. For extended storage, freeze at 20 ℃ in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

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12 - Non Combustible Liquids