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Anti-Grb-2 antibody, Mouse monoclonal
| 产品编号： | 3145906 |
| 规格： | clone GRB-232, purified from hybridoma cell culture |
| 包装规格： | 0.2 ML |
| 产品类别： | 进口试剂 |
| 品牌： | Sigma-Aldrich |
| 优惠价： | 立即咨询 |
基本信息
| MDL number | MFCD00802742 |
| NACRES | NA.44 |
| General description【一般描述】 | Grb2 is an adapter protein with one Src homology 2 (SH2) domain and two SH3 domains that facilitate protein-protein interactions. grb2 is highly conserved across species and is critical for development events such as epithelial morphogenesis, cell motility and vasculogenesis. Grb2 is closely associated with Sos and activates Ras and the MAPK cascade. Grb2 interacts with signalling proteins involved in T-cell development, B-cell activation and development and autoimmunity. Recent reports implicates Grb2 role in several oncogenic signalling pathways that lead to CML and breast cancer. Monoclonal Anti-Grb2 reacts specifically with Grb2 (24 kDa). |
| Immunogen【免疫原】 | synthetic peptide (a.a. 200-217) corresponding to the C-terminal region of human, rat and mouse Grb2. |
| Application【应用】 | Anti-Grb-2 antibody may be used at a working concentration of 1-2 μg/mL for detection by immunoblotting in whole cell extracts of human T cell leukemia, Jurkat cells. Detection is also possible in extracts of murine spermatozoa by immunoblotting at a working dilution of 1:1000. The antibody is suitable for indirect ELISA, immunocytochemistry, immunohistochemistry, immunoprecipitation and protein microarray. |
| Physical form【外形】 | 0.01M 磷酸缓冲盐溶液，pH 7.4，含 15mM 叠氮化钠。 |
| Preparation Note【制备说明】 | Purified from culture supernatant of hybridoma cells grown in a bioreactor. |
产品性质
| Quality Level【质量水平】 | 200 |
| biological source【生物来源】 | mouse |
| conjugate【偶联物】 | unconjugated |
| antibody form【抗体形式】 | purified from hybridoma cell culture |
| antibody product type | primary antibodies |
| clone【克隆】 | GRB-232, monoclonal |
| form【形式】 | buffered aqueous solution |
| mol wt【分子量】 | antigen 24 kDa |
| species reactivity | rat, mouse, human |
| concentration【浓度】 | ~2 mg/mL |
| technique(s) | immunocytochemistry: suitable immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable immunoprecipitation (IP): suitable indirect ELISA: suitable microarray: suitable western blot: 1-2 μg/mL using a whole extract of cultured human acute T-cell leukemia Jurkat cells |
| isotype【同位素/亚型】 | IgG3 |
| UniProt accession no.【UniProt登记号】 | P62993 |
| shipped in【运输】 | dry ice |
| storage temp.【储存温度】 | −20℃ |
| Gene Information | human ... GRB2(2885) mouse ... Grb2(14784) rat ... Grb2(81504) |
产品说明
| Disclaimer【免责声明】 | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
