Monoclonal Anti-MASTL antibody produced in mouse

NA.41

Monoclonal Anti-MASTL (mouse IgG2b isotype) is derived from the hybridoma 2C8/D11 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a fusion protein. Microtubule-Associated Serine/Threonine kinase-Like (MASTL), also known as GREATWALL (GWL) in Drosophila and Xenopus is localized to the nucleus but, during M-phase it is activated and re-localized to the centrosomes.

immunized with a fusion protein of 250aa polypeptide from the region that divides the kinase domain of mouse MASTL

Monoclonal Anti-MASTL antibody produced in mouse may be used in various immunochemical techniques including immunoblotting, flow cytometry and immunofluorescence.

Microtubule-associated serine/Threonine kinase-Like (MASTL) acts as a cell cycle marker of M phase. It has an ability to regulate mitosis entry, anaphase and cytokinesis. In Drosophila MASTL (Greatwall) was initially identified as a kinase essential for chromosome condensation. MASTL enhances cyclin B1-Cdk1-dependent mitotic phosphorylation events. Furthermore, MASTL is also an inhibitor of the tumor suppressor protein Phosphatase 2A (PP2A), and thus maintains the equilibrium between cyclin B-Cdk1 and PP2A. A missense mutation in the MASTL gene leads to the development of autosomal dominant inherited thrombocytopenia.

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

200

mouse

purified immunoglobulin

primary antibodies

2C8/D11, monoclonal

buffered aqueous solution

~100 kDa

monkey, human

~1 mg/mL

flow cytometry: 10-20 μg/test using using human HeLa cells.
immunoblotting: 1-2 μg/mL using whole extracts of human HeLa cells.
immunofluorescence: 10-20 μg/mL using using human HeLa cells.

IgG2b

Q96GX5

dry ice

−20℃

mouse ... Mastl(67121)

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