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Anti-NEZHA antibody, Mouse monoclonal
| 产品编号： | 3149195 |
| 规格： | clone NEZHA-1, purified from hybridoma cell culture |
| 包装规格： | 200 μL |
| 产品类别： | 进口试剂 |
| 品牌： | Sigma-Aldrich |
| 优惠价： | 立即咨询 |
产品别名
Anti-NEZHA antibody, Mouse monoclonal
Anti-Calmodulin regulated spectrin-associated protein family member 3
Monoclonal Anti-NEZHA antibody produced in mouse
Anti-CAMSAP3
Anti-KIAA
基本信息
| NACRES | NA.41 |
| General description【一般描述】 | Monoclonal Anti-NEZHA (mouse IgG1 isotype) is derived from the hybridoma NEZHA-1 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synthetic peptide corresponding to a sequence at the C-terminal region of human NEZHA, conjugated to KLH. Nezha (a deity in Chinese mythology) also known as KIAA1543 and calmodulin-regulated spectrin associated protein, is a 1276 amino acid protein that contains one calponin homology (CH) and two coiled coil (CC1 and 2) domains, as well as one DUF1781 (DUF) domain of unknown function. NEZHA is mapped to human chromosome 19p13. NEZHA/ calmodulin regulated spectrin associated protein family member 3 (CAMSAP3) contains a conserved domain called cholecystokinin (CKK) domain at carboxy-terminal, as well as several coiled-coil regions and an calponin homology domain at amino-terminal. |
| Immunogen【免疫原】 | synthetic peptide corresponding to a sequence at the C-terminal region of human NEZHA, conjugated to KLH. The corresponding sequence is identical in mouse, rat, monkey and dog NEZHA. |
| Application【应用】 | Monoclonal Anti-NEZHA antibody produced in mouse has been used in immunofluorescence staining. Monoclonal Anti-NEZHA antibody produced in mouse has been used in:
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| Biochem/physiol Actions【生化/生理作用】 | CAMSAP3 (calmodulin-regulated spectrin-associated protein 3)/ NEZHA modulates the minus-end dynamics of microtubules. It helps to maintain neuronal polarity. Absence of this gene results in the formation of supernumerary axon. NEZHA/ calmodulin regulated spectrin associated protein family member 3 (CAMSAP3) facilitates translocation of Golgi vesicles in epithelial cells. In addition, it also helps in positioning the apical-to-basal polarity of microtubules in epithelial cells. |
| Physical form【外形】 | 0.01M 磷酸缓冲盐溶液，pH 7.4，含 15mM 叠氮化钠。 |
产品性质
| Quality Level【质量水平】 | 200 |
| biological source【生物来源】 | mouse |
| conjugate【偶联物】 | unconjugated |
| antibody form【抗体形式】 | purified from hybridoma cell culture |
| antibody product type | primary antibodies |
| clone【克隆】 | NEZHA-1, monoclonal |
| form【形式】 | buffered aqueous solution |
| mol wt【分子量】 | antigen ~150 kDa |
| species reactivity | human, canine |
| concentration【浓度】 | ~1.0 mg/mL |
| technique(s) | immunoprecipitation (IP): suitable indirect immunofluorescence: suitable western blot: 2.5-5.0 μg/mL using whole extracts of human SW480 cells |
| isotype【同位素/亚型】 | IgG1 |
| UniProt accession no.【UniProt登记号】 | Q9P1Y5 |
| shipped in【运输】 | dry ice |
| storage temp.【储存温度】 | −20℃ |
| Gene Information | human ... CAMSAP3(57662) mouse ... Camsap3(69697) |
产品说明
| Disclaimer【免责声明】 | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
安全信息
| Storage Class Code【储存分类代码】 | 12 - Non Combustible Liquids |
