广州伟伯科技有限公司
您的位置:首页 > 产品中心 > Monoclonal Anti-Nitric Oxide Synthase, Endothelial antibody produced in mouse
产品搜索:

Monoclonal Anti-Nitric Oxide Synthase, Endothelial antibody produced in mouse

产品编号:3149270
规格:clone NOS-E1, ascites fluid
包装规格:0.2 ML
产品类别:进口试剂
品牌:Sigma-Aldrich
优惠价:立即咨询
产品价格
产品编号包装单位单价(元)国内现货国外库存询价单
31492700.2 ML4730
产品别名

Monoclonal Anti-Nitric Oxide Synthase, Endothelial antibody produced in mouse

Anti-eNOS

基本信息
MDL number
MFCD00675266
NACRES
NA.41
General description【一般描述】
Monoclonal Anti-Nitric Oxide Synthase, endothelial (eNOS) (mouse IgA isotype) is derived from the NOS-E1 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized BALB/c mouse. NOS consists of heme and calmodulin binding sites located within its N-terminal half. It has been localized in many different cell types. Based on molecular mass, subcellular location and Ca2+ dependence, at least three types of NOS have been classified. Type I NOS (150-160 kD protein) is found in neurons and is also called as neuronal NOS (nNOS), brain NOS (bNOS), cerebral NOS, constitutive NOS or Ca2+-regulated NOS (cNOS). Type II (130 kD protein) is found in macrophages and is also known as macrophage NOS (mNOS) or inducible NOS (iNOS). Type III (135 kD protein) is found in endothelial cells and is also called endothelial NOS (eNOS or ecNOS).
The gene encoding endothelial nitric oxide synthase (eNOS) is localized on chromosome 7q35-36 and has 26 exons.
Immunogen【免疫原】
synthetic peptide corresponding to nitric oxide synthase (NOS) from bovine endothelial origin (eNOS, amino acids 1185-1205 with an N-terminally added lysine) conjugated to KLH. The immunogen sequence is highly conserved in human eNOS.
Application【应用】
Monoclonal Anti-Nitric Oxide Synthase, Endothelial (eNOS) has been used:
  • for enzyme linked immunosorbent assay (ELISA)
  • immunoblot
  • immunohistochemistry
  • immunofluorescence.


Monoclonal Anti-Nitric Oxide Synthase, Endothelial antibody produced in mouse has been used in antibody microarray.
Biochem/physiol Actions【生化/生理作用】
NOS catalyses the oxidation of guanidino nitrogen of arginine. The released nitric oxide mediates vasodilatation, neurotransmission, and antimicrobial and anti-tumor activities. NOS is associated with multiple sclerosis and allergic encephalomyelitis.
Endothelial nitric oxide synthase (eNOS) has been studied as a modulator of vascular function and it is involved in the production of nitric oxide. Defects in the enzyme expression have been shown to be associated with coronary artery disease.
产品性质
Quality Level【质量水平】
200
biological source【生物来源】
mouse
conjugate【偶联物】
unconjugated
antibody form【抗体形式】
ascites fluid
antibody product type
primary antibodies
clone【克隆】
NOS-E1, monoclonal
mol wt【分子量】
antigen 135 kDa
contains【包含】
15 mM sodium azide
species reactivity
mouse, human, bovine, rat
technique(s)
microarray: suitable
western blot: 1:3,000 using whole cell extract of cultured bovine lung endothelial cells
isotype【同位素/亚型】
IgA
UniProt accession no.【UniProt登记号】
P29474
shipped in【运输】
dry ice
storage temp.【储存温度】
−20℃
Gene Information
human ... NOS3(4846)
mouse ... Nos3(18127)
rat ... Nos3(24600)
产品说明
Disclaimer【免责声明】
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
安全信息
Storage Class Code【储存分类代码】
12 - Non Combustible Liquids
WGK
WGK 3
广州伟伯科技有限公司 版权所有 CopyRight ©2006-2024, All Rights Reserved
工信部备案号:粤ICP备08114744号   Page Run Time: 0.0095