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Anti-NONO antibody, Mouse monoclonal

产品编号:3149364
规格:clone NC5, purified from hybridoma cell culture
包装规格:200 μL
产品类别:进口试剂
品牌:Sigma-Aldrich
优惠价:立即咨询
产品价格
产品编号包装单位单价(元)国内现货国外库存询价单
3149364200 μL5040
产品别名

Anti-NONO antibody, Mouse monoclonal

Monoclonal Anti-NRB54

Monoclonal Anti-p54(nrb)

Monoclonal Anti-54 kDa nuclear RNA- and DNA-binding protein

Monoclonal Anti-52 kDa subunit

Monoclonal Anti-P54NRB

Monoclonal Anti-NONO antibody produced in mouse

Monoclonal Anti-55 kDa nuclear protein

Monoclonal Anti-DNA-binding p52/p100 complex

Monoclonal Anti-P54

Monoclonal Anti-non-POU domain-containing octamer (ATGCAAAT) binding protein

Monoclonal Anti-NMT55

基本信息
NACRES
NA.41
General description【一般描述】
Monoclonal Anti-NONO (mouse IgG2a isotype) is derived from the hybridoma NC5 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synthetic peptide. Non-POU-domain-containing, octamer binding protein (NONO), also known as p54nrb, is a member of the DBHS (Drosophila behavior, human splicing) protein family. The family also consists of paraspeckle protein 1 (PSPC1) and splicing factor proline- and glutamine-rich (SFPQ). NONO, PSPC1 and SFPQ are the core protein components of paraspeckles.
Specificity【特异性】
Monoclonal Anti-NONO recognizes mouse NONO.
Immunogen【免疫原】
Synthetic peptide corresponding to a sequence at the C-terminal region of mouse NONO, conjugated to KLH.1 The corresponding sequence is identical in rat and differs by a single amino acids in human.
Application【应用】
Monoclonal Anti-NONO antibody produced in mouse has been used in immunohistochemistry and immunoblotting.
Biochem/physiol Actions【生化/生理作用】
Non-POU-domain-containing, octamer binding protein (NONO) complexes with androgen receptor and activates androgen receptor-mediated transcription. NONO is necessary for cAMP-dependent activation of cAMP response element-binding protein (CREB) target genes. It acts as a bridge between the CREB/ transducers of regulated CREB (TORC) complex and RNA polymerase II. NONO associates with Period circadian protein homolog 1 (PERIOD-1) protein and is essential for the maintenance of circadian rhythm. NONO along with the nuclear factor, polypyrimidine tract-binding protein-associated splicing factor (PSF) binds to defective RNA and are capable of their retention.
Physical form【外形】
0.01M 磷酸缓冲盐溶液,pH 7.4,含 15mM 叠氮化钠。
产品性质
Quality Level【质量水平】
200
biological source【生物来源】
mouse
conjugate【偶联物】
unconjugated
antibody form【抗体形式】
purified from hybridoma cell culture
antibody product type
primary antibodies
clone【克隆】
NC5, monoclonal
form【形式】
buffered aqueous solution
species reactivity
rat, mouse
concentration【浓度】
~1.0 mg/mL
technique(s)
indirect immunofluorescence: 1-2 μg/mL using whole extracts of mouse Hepa1-6 cells.
western blot: 0.5-1.0 μg/mL using whole extracts of mouse Hepa1-6 cells.
isotype【同位素/亚型】
IgG2a
UniProt accession no.【UniProt登记号】
Q99K48
shipped in【运输】
dry ice
storage temp.【储存温度】
−20℃
Gene Information
mouse ... Nono(53610)
rat ... Nono(317259)
产品说明
Storage and Stability【储存及稳定性】
For continuous use, store at 2-8℃ for up to one month. For extended storage, freeze at -20℃ in working aliquots. Repeated freezing and thawing,or storage in “frost-free” freezers,is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.
Disclaimer【免责声明】
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
安全信息
Storage Class Code【储存分类代码】
12 - Non Combustible Liquids
WGK
WGK 2
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