Monoclonal Anti-NQO1 antibody produced in mouse

NA.41

This gene is a member of the NAD(P)H dehydrogenase (quinone) family and encodes a cytoplasmic 2-electron reductase. This FAD-binding protein forms homodimers and reduces quinones to hydroquinones. This protein′s enzymatic activity prevents the one electron reduction of quinones that results in the production of radical species. Mutations in this gene have been associated with tardive dyskinesia (TD), an increased risk of hematotoxicity after exposure to benzene, and susceptibility to various forms of cancer. Altered expression of this protein has been seen in many tumors and is also associated with Alzheimer′s disease (AD). Alternate transcriptional splice variants, encoding different isoforms, have been characterized. (provided by RefSeq)
NAD(P)H quinone dehydrogenase 1 (NQO1) is an obligate two-electron reductase. It is a cytoplasmic, homodimeric protein, with one FAD molecule per monomer. This gene is localized to human chromosome 16q23. It shows a wide range of tissue expression.

NQO1 (AAH07659, 1 a.a. ~ 274 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Sequence
MVGRRALIVLAHSERTSFNYAMKEAAAAALKKKGWEVVESDLYAMNFNPIISRKDITGKLKDPANFQYPAESVLAYKEGHLSPDIVAEQKKLEAADLVIFQFPLQWFGVPAILKGWFERVFIGEFAYTYAAMYDKGPFRSKKAVLSITTGGSGSMYSLQGIHGDMNVILWPIQSGILHFCGFQVLEPQLTYSIGHTPADARIQILEGWKKRLENIWDETPLYFAPSSLFDLNFQAGFLMKKEVQDEEKNKKFGLSVGHHLGKSIPTDNQIKARK

NAD(P)H quinone dehydrogenase 1 (NQO1) uses NADH or NADPH as a reducing cofactor and is characterized by its inhibition by dicoumarol. It is thought to confer protection against natural and exogenous quinones. NQO1 is involved in the conversion of benzene derived quinones to less toxic hydroquinones and participates in benzene-associated hematotoxicity. It is involved in the reduction and eventual activation of few chemotherapeutic drugs and environmental carcinogens, such as heterocyclic amines, nitroaromatic compounds and possibly cigarette smoke particulate. NQO1 is up-regulated in serous ovarian carcinoma and this expression is linked with poor prognosis; hence, this gene has potential as a biomarker for the same.

Solution in phosphate buffered saline, pH 7.4

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100

mouse

unconjugated

purified immunoglobulin

primary antibodies

1E3-A6, monoclonal

buffered aqueous solution

human

immunoprecipitation (IP): suitable
indirect ELISA: suitable
indirect immunofluorescence: suitable
western blot: 1-5 μg/mL

IgG1κ

BC007659

P15559

dry ice

−20℃

human ... NQO1(1728)

12 - Non Combustible Liquids

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)