Monoclonal Anti-Opsin antibody produced in mouse

MFCD01865244

NA.41

Photoreceptors are responsible for the initial step in visual processing, converting the signal of photon absorption into synaptic transmission. The visual pigment in vertebrate rods responsible for the absorption of light quanta is rhodopsin (also called opsin). Rhodopsin comprises >95% of the rod outer segments (ROS) intrinsic membrane protein and is a glycoprotein possessing two asparagine-linked oligosaccharide groups at amino acid residues 2 and 15 of the N-terminus. Light is absorbed by rhodopsin and the subsequent conformational change leads to the activation of a cyclic GMP phosphodiesterase through a specific G-protein intermediate, transducin.
Mouse monoclonal clone RET-P1 anti-Opsin antibody recognizes an epitope located in amino acid residues 4-10 at the N-terminus of the rhodopsin molecule. In immunoblotting of rat retina, the antibody labels a closely spaced doublet of 39 kDa and less intense bands of 78 and 115 kDa, representing rhodopsin monomer and aggregates. Higher M.W. bands are stained in pituitary (251 and 288 kDa) and in hypothalamus (269 and 331 kDa) in addition to several lower M.W. bands. The product specifically labels the cell bodies, outer and inner segments (rods but not cones) of rat photoreceptor surface. It reacts with bovine, duck, amphibian, goldfish, rabbit, rat, turtle, tiger salamander, mouse, dove, quail, and human rhodopsin/opsin.
Opsin belongs to rhodopsin receptor. The gene is located on human chromosome 3q22.1. Opsin gene is mainly expressed in rod cells.

rat retinal membranes.

Mouse monoclonal clone RET-P1 anti-Opsin antibody is used to tag opsin/rhodopsin for detection and quantitation by immunocytochemical and immunohistochemical (IHC) techniques such as immunoblotting, immunocytochemistry of cultured cells, immunohistochemistry (paraformaldehyde/glutaraldehyde-fixed, paraformaldehyde perfusion-fixed, frozen sections), immunoelectron microscopy, ELISA, competitive ELISA, and solid phase RIA. It is used as a probe to determine the presence and roles of opsin/rhodopsin in studies of rhodopsin location, functional properties and molecular mechanisms governing rod photoreceptor differentiation.
Monoclonal Anti-Opsin antibody produced in mouse has been used in immunofluorescence and histology.

Mutations in rhodopsin gene are linked to retinitis pigmentosa (RP) and congenital night blindness.

200

mouse

unconjugated

ascites fluid

primary antibodies

RET-P1, monoclonal

antigen 39 kDa by immunoblotting (IB of rat retina produces closely spaced doublet)

15 mM sodium azide

bovine, quail, dove, rat, tiger salamander, amphibian, rabbit, human, goldfish, turtle, mouse, duck

electron microscopy: suitable
immunocytochemistry: suitable using cultured cells
immunohistochemistry (frozen sections): 1:10,000 using frozen sections of rat eye
indirect ELISA: suitable
radioimmunoassay: suitable
western blot: suitable

IgG1

P08100

dry ice

−20℃

human ... RHO(6010)
mouse ... Rho(212541)
rat ... Rho(24717)

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12 - Non Combustible Liquids

WGK 3

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)