Anti-phospho-PAK1 (pThr²¹²) antibody, Mouse monoclonal

MFCD03457569

NA.44

Monoclonal Anti-Phospho-PAK1 (pThr212) (mouse IgG1 isotype) is derived from the PK-18 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synthetic peptide corresponding to amino acids of human PAK1,1 conjugated to keyhole limpet hemocyanin (KLH). PAK1 (p21-activated kinase 1) is homologous to Sacharomyces cerevisiae kinase Ste20. PAK1 (68 kDa),with a C-terminal serine/threonine kinase domain and a N-terminal CRIB (Cdc42/Rac interactive binding) domain. Thr212 localized in flexible region is of PAK1 domain.
p21-activated kinases are a family of highly conserved serine/threonine protein kinases that are activated by small GTPases of Cdc42 and Rac families. There are 2 groups of PAKs in mammals, group A and B. The group A members are three isoforms, PAK1, PAK2, and PAK3 (also known as α-PAK, γ-PAK, and β-PAK, respectively). PAKs are actively involved in regulation of MAPK pathways by phosphorylation of Raf-1 and MEK-1. They also regulate cytoskeletal reorganization by interaction with GTPases. Autophosphorylation of threonine 423 (threonine 402 for PAK2 and threonine 421 for PAK3), catalyzed by Cdc42, is required for activation of PAK. In the neurons, the reorganization of cytoskeleton during the neurite outgrowth is modulated by phosphorylation of PAK1 at Thr²¹² that regulates a specific signalling pathway
Monoclonal Anti-Phospho-PAK1 (pThr²¹²) reacts specifically with human PAK1 phosphorylated at Thr²¹² (68 kDa), and does not detect the unphosphorylated epitope.

synthetic peptide corresponding to amino acids 203-217 (pThr²¹²) of human PAK1.

Anti-phospho-PAK1 (pThr²¹²) may be used for immunoblotting at a working concentration of 0.2-0.4 μg/mL. The antibody is also suitable for indirect ELISA, immunocytochemistry and protein microarray.
Anti-phospho-PAK1 (pThr212) antibody, Mouse monoclonal has been used in:

• enzyme linked immunosorbent assay (ELISA)
• immunoblotting
• immunohistochemistry

p21-activated kinase 1 (PAK1) also regulates cytoskeletal dynamics by decreasing MLCK (myosin light chain kinase) activity and MLC phosphorylation. PAK1 is reported to be a crucial signaling molecule, involved in the transcription factor nuclear factor κB (NFκB) activation by multiple stimuli. PAK1 regulates cell morphology and polarity in eukaryotic cells and activates downstream targets like JNK (c-Jun N-terminal protein kinase) and p38 mitogen-activated protein kinase (MAPK).. The phosphorylation of actin by PAK1 at thr212 helps in regulating growth cone dynamics.

0.01M 磷酸缓冲盐溶液，pH 7.4，含 15mM 叠氮化钠。

200

mouse

unconjugated

purified from hybridoma cell culture

primary antibodies

PK-18, monoclonal

buffered aqueous solution

antigen 68 kDa

mouse, human, rat

~2 mg/mL

immunocytochemistry: suitable
indirect ELISA: suitable
microarray: suitable
western blot: 0.2-0.4 μg/mL using a whole extract of transfected and activated COS-7 (monkey kidney) cells expressing phosphorylated PAK1

IgG1

Q13153

dry ice

−20℃

human ... PAK1(5058)
mouse ... Pak1(18479)
rat ... Pak1(29431)

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12 - Non Combustible Liquids

WGK 3