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Anti-phospho-RasGRP1(pThr184) antibody, Mouse monoclonal
| 产品编号： | 3150189 |
| 规格： | clone p-RasGRP1-1, purified from hybridoma cell culture |
| 包装规格： | 200 μL |
| 产品类别： | 进口试剂 |
| 品牌： | Sigma-Aldrich |
| 优惠价： | 立即咨询 |
产品别名
Anti-phospho-RasGRP1(pThr184) antibody, Mouse monoclonal
Anti-RAS guanyl releasing protein 1 (calcium and DAG-regulated) hRasGRP1
Anti-CALDAG-GEFII
Anti-CALDAG-GEFI
基本信息
| NACRES | NA.41 |
| General description【一般描述】 | Monoclonal Anti-phospho-RasGRP1(pThr184) (mouse IgG2a isotype) is derived from the hybridoma p-RasGRP1-1 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synthetic phospho-peptide corresponding to residues surrounding Thr184 of human RasGRP1 conjugated to KLH. RAS guanyl releasing protein 1 (RasGRP1) is a member of RasGRP family, characterized by the presence of a N-terminal Ras superfamily guanine nucleotide exchange factor (GEF) domain and and C-terminal calcium- and diacylglycerol (DAG)/phorbol ester-binding domains. It is an intracellular signaling protein. RasGRP1 has a N terminal guanine nucleotide exchange factor domain for Ras-like guanosine triphosphatases (GTPases) (RasGEFN/RasGEF), two EF hand motifs and a carboxy-terminal C1 domain. |
| Immunogen【免疫原】 | a synthetic phospho-peptide corresponding to residues surrounding Thr184 of human RasGRP1, conjugated to KLH. The corresponding sequence is identical in rat, mouse, monkey, bovine and canine RasGRP1. |
| Application【应用】 | Monoclonal Anti-phospho-RasGRP1(pThr184) antibody produced in mouse has been used in immunoblotting and immunoprecipitation. |
| Biochem/physiol Actions【生化/生理作用】 | RAS guanyl releasing protein 1 (RasGRP1) functions as a diacylglycerol (DAG)-regulated nucleotide exchange factor specifically activating Ras through the exchange of bound guanosine diphosphate (GDP) for guanosine triphosphate (GTP). It activates the extracellular signal-regulated kinase (Erk)/mitogen-activated protein (MAP) kinase cascade and regulates T-cells and B-cells development, homeostasis and differentiation. |
| Physical form【外形】 | 0.01M 磷酸缓冲盐溶液，pH 7.4，含 15mM 叠氮化钠。 |
产品性质
| Quality Level【质量水平】 | 200 |
| biological source【生物来源】 | mouse |
| conjugate【偶联物】 | unconjugated |
| antibody form【抗体形式】 | purified from hybridoma cell culture |
| antibody product type | primary antibodies |
| clone【克隆】 | p-RasGRP1-1, monoclonal |
| form【形式】 | buffered aqueous solution |
| mol wt【分子量】 | antigen ~90 kDa |
| species reactivity | human |
| concentration【浓度】 | ~1.0 mg/mL |
| technique(s) | immunoprecipitation (IP): suitable western blot: 2-4 μg/mL using whole extracts of HEK-293T cells overexpressing human RasGRP1 |
| isotype【同位素/亚型】 | IgG2a |
| UniProt accession no.【UniProt登记号】 | O95267 |
| shipped in【运输】 | dry ice |
| storage temp.【储存温度】 | −20℃ |
| Gene Information | human ... RASGRP1(10125) mouse ... Rasgrp1(19419) rat ... Rasgrp1(29434) |
产品说明
| Disclaimer【免责声明】 | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
安全信息
| Storage Class Code【储存分类代码】 | 12 - Non Combustible Liquids |
