单克隆抗磷酸丝氨酸

NA.44

Monoclonal Anti-Phosphoserine (mouse IgG1 isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse.

通过 ELISA 和斑点印迹，该抗体以游离氨基酸形式或与载体(如 BSA 或 KLH)的缀合，与磷酸化丝氨酸特异性反应。与非磷酸化丝氨酸、磷酸苏氨酸、磷酸酪氨酸、AMP 或 ATP 无交叉反应。该抗体已用在免疫印迹中定位一些含磷酸丝氨酸蛋白质。由于识别位点的空间位阻，该抗体可能无法识别已知含有磷酸化丝氨酸的某些蛋白质。

磷酸丝氨酸与钉形贝血蓝蛋白(KLH)结合。

Monoclonal Anti-Phosphoserine - Agarose may be used for the immunoprecipitation or immuno-affinity purification of serine phosphoproteins.The antibody may be used for the localization of some phosphoserine containing proteins using the immunoblotting method.

Reversible phosphorylation of proteins is an important post-translational modification that plays a regulatory role in the expression of most proteins in the cells. Reversible phosphorylation at multiple serine, tyrosine and threonine residues mediate numerous signalling pathways in both prokaryotic and Eukaryotic cells. Cellular proteins with phosphorylated serine increase many fold by the activation of serine kinases. Most mitogenic receptor systems such as EGF, PDGF, insulin receptors contain serine/threonine/tyrosine kinase domains that undergo autophosphorylation when receptors bind to the respective ligands. T cell antigen receptor complex or the receptors for some hemopoietic growth factors may stimulate these phosphorylation associated kinases,3 and cells transformed by viral oncogenes contain elevated levels of phosphorylated tyr/ser/thr.
Monoclonal Anti-Phosphoserine may be used for the identification of proteins containing phosphorylated serine both as the free amino acid or when conjugated to carriers such as BSA or KLH. It does not react with non-phosphorylated serine, phosphorylated tyrosine or threonine, AMP or ATP.

Suspension in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

200

mouse

agarose conjugate

purified immunoglobulin

primary antibodies

PSR-45, monoclonal

buffered aqueous solution

immunoprecipitation (IP): suitable

IgG1

wet ice

2-8℃

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12 - Non Combustible Liquids

WGK 3