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抗 磷酸酪氨酸抗体，小鼠单克隆
Anti-Phosphotyrosine antibody, Mouse monoclonal
| 产品编号： | 3150203 |
| 规格： | clone pT-154, purified from hybridoma cell culture |
| 包装规格： | 200 μL |
| 产品类别： | 进口试剂 |
| 品牌： | Sigma-Aldrich |
| 优惠价： | 立即咨询 |
产品别名
抗 磷酸酪氨酸抗体，小鼠单克隆
Anti-Phosphotyrosine antibody, Mouse monoclonal
Phospho-Tyr
p-Tyr
Phospho-tyrosine
基本信息
| MDL number | MFCD00164715 |
| NACRES | NA.44 |
| General description【一般描述】 | Anti-Phosphotyrosine antibody, Mouse monoclonal (mouse IgG2b isotype) is derived from the pT-154 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a tyrosine phosphorylated peptide. Members of protein-tyrosine kinases (PTKs) family consists of extra-cellular domain that is responsible for ligand binding, a trans-membrane domain, modular domain and an intracellular domain. |
| Immunogen【免疫原】 | tyrosine phosphorylated peptide. |
| Application【应用】 | Anti-Phosphotyrosine antibody, Mouse monoclonal has been used in:
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| Biochem/physiol Actions【生化/生理作用】 | Protein phosphorylation is a post-translational modification that regulates signal transduction. Phosphorylation of tyrosine residues in proteins has been associated with oncogenesis, cell growth and survival . Monoclonal Anti-Phosphotyrosine antibody is specific for rat and human proteins/peptides that contain phosphotyrosine residues. As determined by competitive ELISA, the product does not bind to non-phosphorylated tyrosine, phosphorylated serine or threonine, ATP or AMP. Protein-tyrosine kinases (PTKs) are enzymes that catalyze the transfer of Γ-phosphate of ATP to tyrosine residues of protein substrates. The PTKs are responsible for many biological processes like cell cycle, proliferation, oncogenesis and development. They are tightly regulated by other kinases and by autophosphorylation activity. Monoclonal antibodies specific for phosphotyrosine are essential tools for the study of tyrosine phosphorylation in post-translational modification in many biological processes. |
| Physical form【外形】 | 0.01M 磷酸缓冲盐溶液，pH 7.4，含 15mM 叠氮化钠。 |
产品性质
| Quality Level【质量水平】 | 200 |
| conjugate【偶联物】 | unconjugated |
| antibody form【抗体形式】 | purified immunoglobulin |
| antibody product type | primary antibodies |
| clone【克隆】 | pT-154, monoclonal |
| concentration【浓度】 | ~2 mg/mL |
| technique(s) | direct ELISA: suitable immunohistochemistry: suitable microarray: suitable western blot: 2-4 μg/mL using total extract of A431 cells stimulated by EGF |
| isotype【同位素/亚型】 | IgG2b |
| shipped in【运输】 | dry ice |
| storage temp.【储存温度】 | −20℃ |
产品说明
| Disclaimer【免责声明】 | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
安全信息
| Storage Class Code【储存分类代码】 | 12 - Non Combustible Liquids |
