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Monoclonal Anti-Rabbit IgG, Native 小鼠抗
Anti-Rabbit IgG, Native antibody, Mouse monoclonal
产品编号: | 3151153 |
规格: | clone RabT-50, purified from hybridoma cell culture |
包装规格: | 200 μL |
产品类别: | 进口试剂 |
品牌: | Sigma-Aldrich |
优惠价: | 立即咨询 |
产品别名
Monoclonal Anti-Rabbit IgG, Native 小鼠抗
Anti-Rabbit IgG, Native antibody, Mouse monoclonal
基本信息
NACRES | NA.46 |
General description【一般描述】 | This antibody reacts specifically with non-reduced rabbit IgG and does not react with reduced rabbit IgG. Anti-Rabbit IgG, Native antibody, Mouse monoclonal (mouse IgG1 isotype) is derived from the hybridoma RabT-50 produced by the fusion of mouse myeloma cells (NS1) and splenocytes from BALB/c mice. Immunoglobulin G (IgG) is found abundantly in the human serum. It constitutes about 10-20% of the plasma proteins. IgG is composed of glycoproteins, out of which it is 82-96% proteins and 4-18% carbohydrates. It consists of four sub-classes i.e IgG1, IgG2, IgG3, and IgG4. IgG is composed of four polypeptide chains-two heavy chains (γ chains) and two light chains (κ or λ chains) which are linked by inter-chain disulfide bonds. The heavy and the light chains are linked at VH and CH1 domain to form the Fragment antigen binding (Fab) arm. The antigen binds to the V regions of the antibody. |
Specificity【特异性】 | Anti-Rabbit IgG, Native antibody, Mouse monoclonal reacts specifically with non-reduced rabbit IgG and does not react with reduced rabbit IgG. It does not recognize human, monkey, bovine, horse, pig, goat, cat, rat, and chicken IgGs. |
Immunogen【免疫原】 | Enriched fraction of rabbit IgG light chain. |
Application【应用】 | Anti-Rabbit IgG, Native antibody, Mouse monoclonal has been used in:
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Biochem/physiol Actions【生化/生理作用】 | IgG antibody subtype is the most abundant of serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids and provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defense of the neonate against infections. |
Physical form【外形】 | 0.01M 磷酸缓冲盐溶液,pH 7.4,含 15mM 叠氮化钠。 |
产品性质
Quality Level【质量水平】 | 200 |
biological source【生物来源】 | mouse |
conjugate【偶联物】 | unconjugated |
antibody form【抗体形式】 | purified immunoglobulin |
antibody product type | secondary antibodies |
clone【克隆】 | RabT-50, monoclonal |
form【形式】 | buffered aqueous solution |
species reactivity | rabbit |
should not react with | horse, monkey, cat, human, goat, pig, bovine, rat, chicken |
concentration【浓度】 | ~1 mg/mL |
technique(s) | immunocytochemistry: suitable indirect ELISA: suitable western blot: 1-2 μg/mL using 10 ng of non-reduced rabbit IgG (Cat. No. I5006) per lane |
isotype【同位素/亚型】 | IgG1 |
shipped in【运输】 | dry ice |
storage temp.【储存温度】 | −20℃ |
产品说明
Storage and Stability【储存及稳定性】 | For extended storage, freeze at –20 ℃ in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discard if not used within 12 hours. |
Disclaimer【免责声明】 | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
安全信息
Storage Class Code【储存分类代码】 | 12 - Non Combustible Liquids |
Personal Protective Equipment【个人防护装备】 | Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter |