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Monoclonal Anti-WRN 小鼠抗
Anti-WRN antibody, Mouse monoclonal
| 产品编号： | 3154503 |
| 规格： | clone 195C, purified from hybridoma cell culture |
| 包装规格： | 200 μL |
| 产品类别： | 进口试剂 |
| 品牌： | Sigma-Aldrich |
| 优惠价： | 立即咨询 |
产品别名
Monoclonal Anti-WRN 小鼠抗
Anti-WRN antibody, Mouse monoclonal
Anti-RECQL3
Anti-RECQL2
Anti-RECQ3
Anti-Werner syndrome protein
Anti-Werner syndrome helicase
基本信息
| MDL number | MFCD02264220 |
| NACRES | NA.41 |
| General description【一般描述】 | Anti-WRN antibody, Mouse monoclonal, (mouse IgG1 isotype) is derived from the hybridoma 195C produced by the fusion of mouse myeloma cells (p3-NS1/Ag4-1) and splenocytes from BALB/c mice immunized with a recombinant fusion protein fragment of human WRN. Werner syndrome ATP-dependent helicase (WRN) is located on chromosome 8 and is 167 kDa. The WRN protein belongs to the RecQ helicase family of DNA helicases. |
| Immunogen【免疫原】 | recombinant fusion protein fragment of human WRN (amino acids 1074-1432). |
| Application【应用】 | Anti-WRN antibody has been used in
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| Biochem/physiol Actions【生化/生理作用】 | Werner syndrome ATP-dependent helicase (WRN) protein has been demonstrated to possess three known catalytic activities: 3′-5′ helicase, exonuclease and ATPase activities. The C-terminal region of the protein contains a nuclear localization signal (NLS), thus targeting WRN protein to the nucleus, nucleolus and nucleoplasmic foci. The involvement of WRN in multiple DNA metabolic process suggests its function as a tumor suppressor. Mutations in the WRN protein results in Werner syndrome (WS). The epigenetic inactivation of WRN leads to loss of WRN-exonuclease activity, resulting in increased chromosomal instability and hypersensitivity to chemotherapeutic drugs. |
| Physical form【外形】 | 0.01M 磷酸缓冲盐溶液，pH 7.4，含 15mM 叠氮化钠。 |
产品性质
| Quality Level【质量水平】 | 200 |
| biological source【生物来源】 | mouse |
| conjugate【偶联物】 | unconjugated |
| antibody form【抗体形式】 | purified immunoglobulin |
| antibody product type | primary antibodies |
| clone【克隆】 | 195C, monoclonal |
| form【形式】 | buffered aqueous solution |
| mol wt【分子量】 | antigen ~167 kDa |
| species reactivity | human |
| concentration【浓度】 | ~1 mg/mL |
| technique(s) | immunohistochemistry: suitable immunoprecipitation (IP): suitable indirect ELISA: suitable western blot: 2-4 μg/mL using nuclear cell extract of HeLa cells |
| isotype【同位素/亚型】 | IgG1 |
| UniProt accession no.【UniProt登记号】 | Q14191 |
| shipped in【运输】 | dry ice |
| storage temp.【储存温度】 | −20℃ |
| Gene Information | human ... WRN(7486) |
产品说明
| Disclaimer【免责声明】 | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
安全信息
| Storage Class Code【储存分类代码】 | 12 - Non Combustible Liquids |
| WGK | nwg |
| Personal Protective Equipment【个人防护装备】 | Eyeshields, Gloves, multi-purpose combination respirator cartridge (US) |
