MystiCq^® microRNA qPCR Control Primer

RNU6-1 Sanger Database

NA.55

The MystiCq microRNA qPCR Control Primer is an integral part of the MystiCq microRNA qPCR Assay System. It has been designed to target specific small RNAs with the MystiCq Universal PCR primer and the MystiCq microRNA SYBR Green qPCR ReadyMix on cDNA templates generated by the MystiCq microRNA cDNA Synthesis Mix.

U6 small nuclear 1 (RNU6-1) gene primer has been used as a positive control for qPCR.

Features and Benefits

• Proprietary design features of assay primers allow for specificity towards mature microRNAs vs. precursor-microRNAs
• Extensive design and testing of oligo-dT adapter primer to incorporate complementary Universal PCR Primer sequence
• No self-complementarity or primer dimer artifacts with the Universal PCR Primer
• Optimized primer Tm designed to match the Universal PCR Primer
• Universal cycling conditions ensures robust amplification for all assays in profiling experiments
• Optimized PCR product Tm (75-78° C)

Detects RNU6-1 and RNU6-2. Compatible with mouse and rat.

GenBank is a registered trademark of United States Department of Health and Human Services
MystiCq is a registered trademark of Qiagen Beverly Inc.

liquid

GUGCUCGCUUCGGCAGCACAUAUACUAAAAUUGGAACGAUACAGAGAAGAUUAGCAUGGCCCCUGCGCAAGGAUGACACGCAAAUUCGUGAAGCGUUCCAUAUUUU

~76.5 ℃

NR_004394.1

wet ice

−20℃

human ... RNU6-1(26827)

12 - Non Combustible Liquids

WGK 1