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产品性质
| biological source【生物来源】 | mouse connective tissue |
| growth mode【生长模式】 | Adherent |
| karyotype【核型】 | 2n = 40 |
| morphology【形态学】 | Fibroblast |
| products【产品】 | Not specified |
| receptors【受体】 | Not specified |
| technique(s) | cell culture | mammalian: suitable |
| shipped in【运输】 | dry ice |
| storage temp.【储存温度】 | −196℃ |
基本信息
| Cell Line Origin【细胞系来源】 | Mouse C3H/An connective tissue |
| Cell Line Description【细胞系描述】 | From parental strain L which was derived from normal subcutaneous areolar and adipose tissue of 100 day old male C3H/An. Used in development of replicate culture techniques, chemically defined media, fluid suspension culture methods and virus assays. Susceptible to pseudorabies virus and vesicular stomatitis (Indiana strain) virus. |
| Application【应用】 | Replication, biochemical, neoplastic transformation, nutritional, metabolic enzyme, morphological, chromosome, immunologic, radiation, toxicologic and virus studies |
| Culture Medium【培养基】 | EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS). |
| Subculture Routine【传代培养常规】 | Split sub-confluent cultures (70-80%) 1:3 to 1:10 i.e. seeding at 1-3x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37℃. Freeze in 10% glycerol/90% FBS. After resuscitation cells may take up to 4 days to establish growth. |
| Other Notes【其他说明】 | Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information. |
