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pBICEP-CMVTM-3 Expression Vector

产品编号:3179277
包装规格:20 μG
产品类别:进口试剂
品牌:Sigma-Aldrich
优惠价:立即咨询
产品价格
产品编号包装单位单价(元)国内现货国外库存询价单
317927720 μG9070
产品性质
Quality Level【质量水平】
200
shipped in【运输】
dry ice
storage temp.【储存温度】
−20℃
packaging【包装】
20 μg in poly bottle
基本信息
General description【一般描述】
The pBICEP-CMV-3 bicistronic expression vector is a 5.3 kb derivative of pCMV5 used for transient or stable co-expression of an N-terminal 1´ FLAGÒ fusion protein and a selection gene in mammalian cells.
Application【应用】
For transient, cytoplasmic expression of an N-terminal FLAG® fusion and a second gene of interest or selection marker from bicistronic mRNA. Two genes can be cloned into MCS1 and MCS2 for transcription of a single message from the CMV promoter. This vector is useful for protein-protein interaction studies, multi-subunit proteins, and cloning a selection marker of choice.
Biochem/physiol Actions【生化/生理作用】
The presence of two multiple cloning sites not only allows the user to clone a target gene, but a selectable marker of their choice, as well. The promoter-regulatory region of the human cytomegalovirus immediate early promoter drives transcription of the FLAG-fusion construct along with a downstream selection gene. The EMCV IRES region controls translation of the selection gene by recruiting the ribosomal subunits for cap-independent translational initiation. Depending on the mode of selection, stable transfectants can be generated by transfection using the appropriate ESCORTä product line specific for the cell type.
Other Notes【其他说明】
您可在我们的FLAG®文献门户上浏览更多应用文献。
Legal Information【法律信息】
FLAG is a registered trademark of sigma-aldrich Co. LLC
pBICEP-CMV is a trademark of sigma-aldrich Co. LLC
安全信息
Storage Class Code【储存分类代码】
12 - Non Combustible Liquids
WGK
WGK 3
Personal Protective Equipment【个人防护装备】
Eyeshields, Gloves
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