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pT7-FLAGTM-2 Expression Vector

产品编号:3210695
包装规格:10 μG
产品类别:进口试剂
品牌:Sigma-Aldrich
优惠价:立即咨询
产品价格
产品编号包装单位单价(元)国内现货国外库存询价单
321069510 μG4840
基本信息
NACRES
NA.85
General description【一般描述】
pT7-FLAG-2 is a 4.8 kb E . coli expression vector used for cloning and cytoplasmic expression of a properly inserted open reading frame as a C-terminal FLAG® fusion protein containing the FLAG epitope (DYKDDDDK). The promoter region of the very strong phage T7 promoter drives transcription of ORFFLAG fusion constructs. This vector requires the use of E. coli cells containing a source of the T7 polymerase, such as BL21(DE3)T1R cells, Catalog Number B2935.

The C-terminal FLAG fusion protein may be detected using Monoclonal ANTI-FLAG?M2, Catalog Number
F3165, and purified using the ANTI-FLAG M2 Affinity Gel, Catalog Number A2220.

Vector Maps and Sequences
Components【组分】
  • pT7-FLAG-2 Expression Vector, 10 μg (P6867) is supplied as 0.5 mg/ml in 10 mM Tris-HCl (pH 8.0) with 1 mM EDTA.
  • pT7-FLAG-2-BAP Control Vector 1 μg ( P7117) is supplied as 0.5 mg/ml in 10 mM Tris-HCl (pH 8.0) with 1 mM EDTA.
Legal Information【法律信息】
FLAG is a registered trademark of sigma-aldrich Co. LLC
pT7-FLAG is a trademark of sigma-aldrich Co. LLC
产品性质
Quality Level【质量水平】
200
grade【等级】
for molecular biology
shipped in【运输】
dry ice
storage temp.【储存温度】
−20℃
安全信息
Storage Class Code【储存分类代码】
12 - Non Combustible Liquids
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