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REDAccuTaq® LA DNA Polymerase
产品编号: | 3214675 |
规格: | High fidelity Taq with inert dye, 10X buffer included |
包装规格: | 250 UNITS |
产品类别: | 进口试剂 |
品牌: | Sigma-Aldrich |
优惠价: | 立即咨询 |
基本信息
MDL number | MFCD01092095 |
NACRES | NA.55 |
General description【一般描述】 | REDAccuTaq® long and accurate (LA) DNA Polymerase is a blend of Taq DNA polymerase and a small amount of thermostable proofreading polymerase that exhibits 3′→5′ exonuclease and an inert red dye that acts as a tracer and loading buffer. It is suitable for amplifying complex DNA templates like human genomic DNA. REDAccuTaq LA DNA polymerase allows for quick recognition in high throughput applications as well as direct loading of amplification products onto agarose gels for electrophoresis. The inert red dye has no effect on automated sequencing, restriction enzyme digestion, ligation, or other downstream manipulations. However, the PCR product is easily separated from the dye by standard purification methods. |
Application【应用】 | REDAccuTaq® LA DNA Polymerase produce long DNA amplicons for:
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Features and Benefits【特点和优势】 |
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Unit Definition【单位定义】 | One unit incorporates 10 nmol of total dNTPs into acid-precipitable DNA in 30 min. at 74 ℃. |
Other Notes【其他说明】 | Store REDAccuTaq LA DNA Polymerase and AccuTaq LA 10x Buffer at –20 ℃. |
Legal Information【法律信息】 | 本产品的使用受到如下一项或多项美国专利及其对应的境外专利声明保护:5,789,224, 5,618,711, 6,127,155以及与届满的美国专利号5,079,352对应的境外专利声明。购买本产品,即相当于依照境外的专利声明获得了一份受限制、不可转让的使用许可,即将此等数量的产品用于购买者内部的研究用途。我们未明确表示、暗示或以禁止反言的形式授予您任何其他专利声明下的权利、进行任何专利方法申请的权利、进行任何形式的商业服务的权利,包括但不限于出于收费或其他商业考虑而报告购买者的研究活动结果的权利。本产品仅适合于研究用途。如需用于Roche专利许可的诊断用途,需另外征得Roche许可。有关购买许可的更多信息,可联系Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA获取。 REDAccuTaq is a registered trademark of sigma-aldrich Co. LLC |
产品性质
Quality Level【质量水平】 | 200 |
form【形式】 | liquid |
usage【用途】 | sufficient for 250 reactions |
feature【特点】 | Long & Accurate PCR dNTPs included: no hotstart: no |
concentration【浓度】 | 1 unit/μL |
technique(s) | PCR: suitable |
color【颜色】 | red |
input | purified DNA |
shipped in【运输】 | wet ice |
storage temp.【储存温度】 | −20℃ |
packaging【包装】 | The enzyme is provided with an optimized 10× reaction buffer for enhanced amplification of complex templates. |
安全信息
Storage Class Code【储存分类代码】 | 12 - Non Combustible Liquids |
WGK | WGK 3 |
Personal Protective Equipment【个人防护装备】 | Eyeshields, Gloves |