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REDAccuTaq® LA DNA Polymerase

产品编号:3214675
规格:High fidelity Taq with inert dye, 10X buffer included
包装规格:250 UNITS
产品类别:进口试剂
品牌:Sigma-Aldrich
优惠价:立即咨询
产品价格
产品编号包装单位单价(元)国内现货国外库存询价单
3214675250 UNITS2940
基本信息
MDL number
MFCD01092095
NACRES
NA.55
General description【一般描述】
REDAccuTaq® long and accurate (LA) DNA Polymerase is a blend of Taq DNA polymerase and a small amount of thermostable proofreading polymerase that exhibits 3′→5′ exonuclease and an inert red dye that acts as a tracer and loading buffer. It is suitable for amplifying complex DNA templates like human genomic DNA.
REDAccuTaq LA DNA polymerase allows for quick recognition in high throughput applications as well as direct loading of amplification products onto agarose gels for electrophoresis. The inert red dye has no effect on automated sequencing, restriction enzyme digestion, ligation, or other downstream manipulations. However, the PCR product is easily separated from the dye by standard purification methods.
Application【应用】
REDAccuTaq® LA DNA Polymerase produce long DNA amplicons for:
  • Mutation analysis
  • Cloning genes
  • DNA sequencing
  • cDNA library generation
  • Amplicon sequencing
Features and Benefits【特点和优势】
  • Increased fidelity, up to 6.5× that of Taq DNA polymerase
  • Efficiently and accurately produce amplicons up to 22 kb on genomic templates and up to 40 kb on less complex templates such as lambda or bacterial DNA
  • Visual confirmation that enzyme has been added, and proper mixing has occurred
  • No additional loading dyes needed. A post-reaction aliquot can be directly used on an agarose gel for electrophoresis
Unit Definition【单位定义】
One unit incorporates 10 nmol of total dNTPs into acid-precipitable DNA in 30 min. at 74 ℃.
Other Notes【其他说明】
Store REDAccuTaq LA DNA Polymerase and AccuTaq LA 10x Buffer at –20 ℃.
Legal Information【法律信息】
本产品的使用受到如下一项或多项美国专利及其对应的境外专利声明保护:5,789,224, 5,618,711, 6,127,155以及与届满的美国专利号5,079,352对应的境外专利声明。购买本产品,即相当于依照境外的专利声明获得了一份受限制、不可转让的使用许可,即将此等数量的产品用于购买者内部的研究用途。我们未明确表示、暗示或以禁止反言的形式授予您任何其他专利声明下的权利、进行任何专利方法申请的权利、进行任何形式的商业服务的权利,包括但不限于出于收费或其他商业考虑而报告购买者的研究活动结果的权利。本产品仅适合于研究用途。如需用于Roche专利许可的诊断用途,需另外征得Roche许可。有关购买许可的更多信息,可联系Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA获取。
REDAccuTaq is a registered trademark of sigma-aldrich Co. LLC
产品性质
Quality Level【质量水平】
200
form【形式】
liquid
usage【用途】
sufficient for 250 reactions
feature【特点】
Long & Accurate PCR
dNTPs included: no
hotstart: no
concentration【浓度】
1 unit/μL
technique(s)
PCR: suitable
color【颜色】
red
input
purified DNA
shipped in【运输】
wet ice
storage temp.【储存温度】
−20℃
packaging【包装】
The enzyme is provided with an optimized 10× reaction buffer for enhanced amplification of complex templates.
安全信息
Storage Class Code【储存分类代码】
12 - Non Combustible Liquids
WGK
WGK 3
Personal Protective Equipment【个人防护装备】
Eyeshields, Gloves
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