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REDTaq® 基因组 DNA 聚合酶

REDTaq® Genomic DNA Polymerase
产品编号:3214703
规格:without MgCl2
包装规格:250 UNITS,1000 UNITS,2500 UNITS
产品类别:进口试剂
品牌:Sigma-Aldrich
优惠价:立即咨询
产品价格
产品编号包装单位单价(元)国内现货国外库存询价单
3214703250 UNITS1100
32147031000 UNITS3770
32147032500 UNITS7650
产品别名

REDTaq® 基因组 DNA 聚合酶

REDTaq® Genomic DNA Polymerase

基本信息
MDL number
MFCD01635818
NACRES
NA.55
General description【一般描述】
REDTaq® Genomic DNA Polymerase is a unique blend of Taq DNA Polymerase with an inert red dye. This special formulation is designed to provide enhanced amplification of more complex or genomic templates. REDTaq Genomic DNA Polymerase is highly sensitive, produces increased yields and is capable of generating longer product lengths. It has all the advantages of REDTaq DNA polymerase, such as easy visualization of enzyme addition and complete reaction mixing, and direct loading to an agarose gel. The inert red dye does not effect automated or manual sequencing, restriction digestions or other downstream applications. The dye can easily removed by any standard purification method.
Application【应用】
REDTaq® Genomic DNA Polymerase has been used as a component of the polymerase chain reaction (PCR) mix for PCR amplification. It has also been used as a component of the preamplification mix for PCR in amplified fragment length polymorphism (AFLP) analysis of Fusarium oxysporum sp. isolates.
Features and Benefits【特点和优势】
  • Enhanced amplification on genomic and difficult DNA templates
  • Same great performance as Taq DNA Polymerase in a more convenient format for high throughput applications
  • Quick recognition and confirmation of appropriate mixing
  • No loading buffers or tracking dyes necessary. Sample can be taken directly from reaction and loaded onto an agarose gel
  • The samples can be re-amplified as in nested PCR
  • The red dye migrates faster than bromophenol blue
  • Greater consistency across reactions due to proper mixing
Components【组分】
Includes 10× PCR reaction buffer without MgCl2 and a separate tube of 25 mM MgCl2
Unit Definition【单位定义】
在74℃下,一个单位可在30分钟内将10 nM总dNTP掺入酸可沉淀的DNA中。
Legal Information【法律信息】
本产品的使用涵盖在以下一项或多项美国专利以及美国以外的相应专利要求中:美国 8,404,464 和美国7,972,828。本产品的采购包括上述专利要求下的有限、不可转让的诉讼豁免。
REDTaq is a registered trademark of sigma-aldrich Co. LLC
产品性质
Quality Level【质量水平】
200
form【形式】
liquid
usage【用途】
sufficient for 1000 reactions
sufficient for 250 reactions
sufficient for 2500 reactions
feature【特点】
Difficult Templates/Specialty Enzymes PCR
dNTPs included: no
hotstart: no
concentration【浓度】
1 unit/μL
technique(s)
PCR: suitable
color【颜色】
red
input
purified DNA
application(s)
agriculture
shipped in【运输】
wet ice
storage temp.【储存温度】
−20℃
安全信息
Storage Class Code【储存分类代码】
12 - Non Combustible Liquids
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