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RPMI-1640 培养基
RPMI-1640 Medium
产品编号: | 3215842 |
规格: | Modified, with |
包装规格: | 10 X 1 L,10 L |
产品类别: | 进口试剂 |
品牌: | Sigma-Aldrich |
优惠价: | 立即咨询 |
产品别名
RPMI-1640 培养基
RPMI-1640 Medium
基本信息
MDL number | MFCD00217820 |
NACRES | NA.75 |
Components【组分】 | phenol red: no NaHCO3: no L-glutamine: yes sodium pyruvate: no HEPES: no |
Application【应用】 | RPMI-1640培养基被用于以下应用:
RPMI 1640 Medium was developed at Roswell Park Memorial Institute in 1966 by Moore and his co-workers. A modification of McCoy′s 5A Medium, it was formulated to support lymphoblastoid cells in suspension culture, but it has since been shown to support a wide variety of cells that are anchorage dependent. Originally intended to be used with a serum supplement, RPMI 1640 has been shown to support several cell lines in the absence of serum. It has also been widely used in fusion protocols and in the growth of hybrid cells. |
Quantity【数量】 | 按照每升培养基10.4克粉末配制。 |
Reconstitution【重悬】 | 补充2.0 g/L碳酸氢钠。 |
Other Notes【其他说明】 | 酚红会干扰一些细胞在克隆密度下的生长。该培养基适用于干细胞培养或低密度的细胞培养。也推荐用于体外诊断。 |
产品性质
sterility【无菌性】 | sterile |
form【形式】 | powder |
technique(s) | cell culture | mammalian: suitable |
web metadata keyword.default | classic media, classical media, FBS, lymphoblastoid cells, cell fusion, hybrid cells |
shipped in【运输】 | ambient |
storage temp.【储存温度】 | 2-8℃ |
安全信息
Storage Class Code【储存分类代码】 | 11 - Combustible Solids |
WGK | WGK 1 |