^®缓冲剂

NA.25

Open the foil pouch, dissolve contents in 900 mL of water; add additional water to a final volume of 1 liter to make a 0.1 M solution at a temperature of 25 ℃. No pH adjustment is necessary. Guaranteed accuracy: ±0.1 pH units.

Tris has a significant temperature coefficient, so select the mixture to give the desired final pH at the desired temperature.
From 5 ℃ to 25 ℃, the pH decreases an average of 0.03 pH units per ℃.
From 25 ℃ to 37 ℃, the pH decreases an average of 0.025 pH units per ℃.

If a different concentration is desired, note that there is a concentration effect on the pH of the solution.
该产品经细胞培养测试。它还经过了低内毒素水平检测并分析了是否已去除核酸酶和蛋白酶。

This product is a pre-mixed combination of Trizma base and Trizma HCl in convenient foil pouches that can be used to prepare a common pH value for Tris buffer.
某些pH电极不能使用Tris缓冲液提供准确的结果。

Trizma溶液可高压灭菌。

Trizma is a registered trademark of sigma-aldrich Co. LLC

200

BioPerformance Certified

crystalline powder

average M_w 141.8

cell culture | mammalian: suitable

DNase, RNase, Protease, NICKase, none detected
endotoxin, tested
total aerobic microbial count, tested
≤0.05 A₂₉₀
≤0.5% water (Karl Fischer)
≤5 ppm Heavy metals (as Pb)

7.71 (37 ℃, 0.1 M)(lit.)
8.00 (25 ℃, 0.1 M)(lit.)
8.0
8.58 (5 ℃, 0.1 M)(lit.)

7.0 - 9.0

diagnostic assay manufacturing

简易锡箔袋包装

11 - Combustible Solids

WGK 1

dust mask type N95 (US), Eyeshields, Gloves