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Trypsin Agarose
| 产品编号： | 3256479 |
| 规格： | buffered aqueous suspension, from bovine pancreas trypsin |
| 包装规格： | 25 UNITS，50 UNITS |
| 产品类别： | 进口试剂 |
| 品牌： | Sigma-Aldrich |
| 优惠价： | 立即咨询 |
基本信息
| Enzyme Commission (EC) Number【酶学委员会(EC)编号】 | 3.4.21.4 ( BRENDA | IUBMB ) |
| MDL number | MFCD00082094 |
| eCl@ss | 42020142 |
| NACRES | NA.54 |
| General description【一般描述】 | Trypsin Agarose is an insoluble enzyme product. It is produced by reacting a conventional "soluble" enzyme (trypsin) with an inert base (agarose). This insoluble conjugate retains the activity of the original enzyme. Trypsin bound to agarose are highly stable and maintain denaturing conditions for longer time than the soluble trypsin. The trypsin molecule has two domains: one is related to the enzyme active site and the tryptophan residues; the other is related to the 8-anilinonaphthalene-1-sulfonate binding. |
| Application【应用】 | A very active and very stable trypsin agarose derivative has been used to optimize the design of the synthesis of a model dipeptide, benzoylarginine leucinamide. Trypsin has also been used in a study to investigate protonation-state determination in proteins using high-resolution X-ray crystallography. Trypsin Agarose has been used for enzymatic hydrolysis of prolamins and gliadin to generate peptides. |
| Other Notes【其他说明】 | Insolubilized |
| Unit Definition【单位定义】 | One unit will hydrolyze 1.0 μmole of BAEE per min at pH 8.0 at 30 ℃ (titrimetric assay). |
| Physical form【外形】 | Suspension in approx. 10 mM acetic acid, pH 3.2 |
产品性质
| Quality Level【质量水平】 | 200 |
| biological source【生物来源】 | bovine pancreas (trypsin) |
| form【形式】 | buffered aqueous suspension |
| concentration【浓度】 | ≥15 units/mL (packed gel) |
| extent of labeling【标记范围】 | ≥15 units per mL packed gel |
| matrix【基质】 | cross-linked beaded agarose |
| shipped in【运输】 | wet ice |
| storage temp.【储存温度】 | 2-8℃ |
| packaging【包装】 | 50 units in glass bottle |
安全信息
| Pictograms【象形图】 | GHS07 |
| Signal word【警示用语：】 | Warning |
| Hazard Statements | H315 - H317 - H319 - H335 |
| Precautionary Statements | P280 - P302 + P352 - P305 + P351 + P338 |
| Hazard Classifications【危险分类】 | Eye Irrit. 2 - Skin Irrit. 2 - Skin Sens. 1 - STOT SE 3 |
| Target Organs【靶器官】 | Respiratory system |
| Storage Class Code【储存分类代码】 | 10 - Combustible liquids |
| WGK | WGK 3 |
| Personal Protective Equipment【个人防护装备】 | Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter |
