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胰蛋白酶-琼脂糖
Trypsin-Agarose
| 产品编号： | 3256480 |
| 规格： | buffered aqueous suspension, from bovine pancreas trypsin |
| 包装规格： | 25 UNITS，50 UNITS，100 UNITS |
| 产品类别： | 进口试剂 |
| 品牌： | Sigma-Aldrich |
| 优惠价： | 立即咨询 |
产品别名
胰蛋白酶-琼脂糖
Trypsin-Agarose
基本信息
| MDL number | MFCD00082094 |
| eCl@ss | 42020142 |
| NACRES | NA.54 |
| General description【一般描述】 | The trypsin molecule has two domains: one is related to the enzyme active site and the tryptophan residues; the other is related to the 8-anilinonaphthalene-1-sulfonate binding. |
| Application【应用】 | An experimental approach using SDS-PAGE, in-gel trypsin digestion and proteomic analysis identified five endosomal proteins. Trypsin cleavage of the high-risk HPV-16 E5 protein (16E5) generates a unique four-amino acid C-terminal peptide that serves as a marker for E5 expression in transfected cells and epithelial cell lines containing integrated and episomal HPV-16 DNA. |
| Unit Definition【单位定义】 | One unit will hydrolyze 1.0 μmole of BAEE per min at pH 8.0 at 30 ℃ (titrimetric assay). |
| Physical form【外形】 | Suspension in approx. 10 mM acetic acid, pH 3.2, containing preservative |
| Preparation Note【制备说明】 | TPCK-treated |
产品性质
| Quality Level【质量水平】 | 200 |
| biological source【生物来源】 | bovine pancreas (trypsin) |
| form【形式】 | buffered aqueous suspension |
| concentration【浓度】 | ≥15 units/mL (packed gel) |
| extent of labeling【标记范围】 | ≥15 units per mL (packed gel) |
| matrix【基质】 | beaded agarose |
| storage temp.【储存温度】 | 2-8℃ |
安全信息
| Pictograms【象形图】 | GHS07 |
| Signal word【警示用语：】 | Warning |
| Hazard Statements | H317 |
| Precautionary Statements | P261 - P272 - P280 - P302 + P352 - P333 + P313 - P362 + P364 |
| Hazard Classifications【危险分类】 | Skin Sens. 1 |
| Storage Class Code【储存分类代码】 | 10 - Combustible liquids |
| WGK | WGK 3 |
