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WRL 68 (HeLa derivative)

产品编号:3260272
规格:89121403
包装规格:20μl,5μg
产品类别:进口试剂
优惠价:立即咨询
产品价格
产品编号包装单位单价(元)国内现货国外库存询价单
326027220μl4470
32602725μg4630
32602725μg5400
产品性质
biological source【生物来源】
human cervix
growth mode【生长模式】
Adherent
karyotype【核型】
Not specified
morphology【形态学】
Epithelial-like
products【产品】
Not specified
receptors【受体】
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)【相关疾病】
cancer
shipped in【运输】
dry ice
storage temp.【储存温度】
−196℃
基本信息
Cell Line Origin【细胞系来源】
Human cervix carcinoma
Cell Line Description【细胞系描述】
The human hepatic cell line WRL-68 exhibits a morphology similar to hepatocytes and hepatic primary cultures. Cells have been shown to secrete albumin and α-feto protein and express liver specific enzymes such as alanine amino transferase, aspartate amino transferase, gamma-glutamyl transpeptidase and alkaline phosphatase. Previously contaminated with Mycoplasma; treated and cured at ECACC. This cell line was found to be indistinguishable from HeLa by STR PCR DNA profiling. Therefore, the cell line should be considered as derived from HeLa. Ethnicity: Black.
Application【应用】
In vitro hepatic model
DNA Profile【DNA图谱分析】
STR-PCR Data: Amelogenin: X
CSF1PO: 9,10
D13S317: 12,14
D16S539: 9,10
D5S818: 11,12
D7S820: 8,12
THO1: 7
TPOX: 8,12
vWA: 16,18
Culture Medium【培养基】
EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).
Subculture Routine【传代培养常规】
Split sub-confluent cultures (70-80%) 1:2 to 1:6 i.e. seeding at 1-5x10,000 cells/cm2 using 0.25% trypsin; 5% CO2; 37℃.
Other Notes【其他说明】
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
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