Anti-Actin, N-terminal antibody produced in rabbit

MFCD00145889

NA.41

Actin is a cytoskeletal protein that regulates cell motility, secretion, phagocytosis and cytokinesis. The NH2-terminal of actin may function as an antigen. This terminal may also modulate actin interactions and may associate with proteins such as myosin.

Actin is one of the most conserved eukaryotic proteins, which is expressed in mammals and birds as at least six major different isoforms. Four of them represent the differentiation markers of muscle tissues and two are found practically in all non-muscle cells. Actin isoforms show >90% overall sequence homology, but only 50-60% homology in their 18 N-terminal residues. Actin is present in cells both as a globular monomer (G-actin) and as a polymer in filamentous actin (F-actin) that participates in the formation of a variety of stable and labile structures. The presence of actin in cell nuclei and cell membranes has been reported.

synthetic actin N-terminal nonapeptide conjugated to KLH.

Protein lysates generated from embryonic mouse limb tissue was analyzed by western blot using actin as the loading control. Actin was detected using rabbit anti-actin at 1:8000. Actin detected by a rabbit anti-actin antibody was used as a loading control for MCF7 cell lysates . Actin was detected at 42 kDa.
Anti-Actin, N-terminal antibody produced in rabbit has been used in

• immunoblotting
• quantitative chromatin immunoprecipitation (qChIP)

Rabbit anti-actin, N-terminal antibody can be used for western blotting assays. The antibody can also be used for immunocytochemistry at 1-2μg/mL using cultured chicken fibroblasts. Furthermore, the antibody is suitable for use in immunohistochemistry at a concentration of 2-4μg/mL using sections of human appendix, mouse heart, and frog skeletal muscle.

Actin and myosin are constituents of many cell types and are involved in a myriad of cellular processes including maintenance of cell shape, locomotion, secretion, cytoplasmic streaming, phagocytosis, and cytokinesis. The N-terminal domain of actin appears to be a major antigenic region of the molecule. Different isoforms can coexist in the same cell but are differentially regulated, and in most cases cannot substitute for each other functionally.

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% BSA and 15 mM sodium azide.

To view an Actin antibody selection guide, please visit www.sigmaaldrich.com/actin.

rabbit

200

unconjugated

affinity isolated antibody

primary antibodies

polyclonal

buffered aqueous solution

antigen 42 kDa

mouse, frog, rat, human, chicken

antibody small pack of 25 μL

~0.5 mg/mL

immunocytochemistry: 1-2 μg/mL using cultured chicken fibroblasts
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 2-4 μg/mL using sections of human appendix, mouse heart, and frog skeletal muscle
indirect immunofluorescence: suitable
western blot: 0.5-1 μg/mL using whole extract of the human epitheloid carcinoma HeLa cell line.
western blot: 2-4 ng/mL using whole extract of rat skeletal muscle

P63261

wet ice

−20℃

human ... ACTA1(58), ACTA2(59), ACTB(60), ACTC1(70), ACTG1(71), ACTG2(72)
mouse ... Acta1(11459), Acta2(11475), Actb(11461), Actc1(11464), Actg1(11465), Actg2(11468)
rat ... Acta1(29437), Acta2(81633), Actb(81822), Actc1(29275), Actg1(287876), Actg2(25365)

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