Anti-Apolipoprotein J antibody produced in goat

MFCD01092275

Apolipoprotein J is a ubiquitously expressed glycoprotein that is involved in the differentiation and proliferation of cells. Apolipoprotein J in conjunction with apolipoprotein E may affect the onset of amyloid-beta deposition, and also may be involved in the local toxicity associated with these deposits. Hence, apolipoprotein J may function as a cerebrospinal fluid marker for Alzheimer′s disease.
Goat anti-apolipoprotein J antibody recognizes human apolipoprotein J and has negligible cross-reactivity with Type A-I, A-II, B, C-I, C-II, C-III and E apolipoproteins.

Apolipoprotein Type J isolated from human plasma by density gradient centrifugation followed by HPLC purification.

HEK293 cells were transfected with either an expression vector to over expression Clusterin or siRNA to knock down expression of Clusterin. Clusterin levels were monitored by western blot using goat anti-Clusterin antibody at 1:1000.
Goat anti-apolipoprotein J antibody can be used for immunohistochemistry applications using formalin-fixed, paraffin-embedded sections at a concentration ranging from 1:50-1:200. The antibody is also suitable for use in ELISA.

Solution in 0.1 M sodium borate, 0.075 M sodium chloride, 0.005 M EDTA, pH 8.0, and 0.01% sodium azide as preservative.

200

goat

unconjugated

affinity isolated antibody

primary antibodies

polyclonal

buffered aqueous solution

human

ELISA: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:50-1:200
western blot: 1:5,000-1:10,000

P10909

dry ice

2-8℃

human ... CLU(1191)

GHS08

Warning

H361d

P201 - P202 - P280 - P308 + P313 - P405 - P501

Repr. 2

12 - Non Combustible Liquids

WGK 3

Eyeshields, Gloves, type ABEK (EN14387) respirator filter