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Anti-Calreticulin antibody produced in rabbit
产品编号: | 3350677 |
规格: | IgG fraction of antiserum, buffered aqueous solution |
包装规格: | 0.2 ML |
产品类别: | 进口试剂 |
品牌: | Sigma-Aldrich |
优惠价: | 立即咨询 |
基本信息
MDL number | MFCD01092536 |
General description【一般描述】 | The gene for calreticulin (CALR) is located on the human chromosome 19p13.13. The encoded protein is a predominantly conserved calcium-binding chaperone, which is localized primarily to the lumen of the endoplasmic reticulum (ER). Calreticulin comprises three domains, a globular N-domain, a proline-rich P-domain, and a highly acidic C-domain. |
Specificity【特异性】 | Anti-Calreticulin recognizes human and dog calreticulin (55-60 kDa). |
Immunogen【免疫原】 | synthetic peptide corresponding to the C-terminus of human calreticulin (amino acids 401-417). |
Application【应用】 | Anti-Calreticulin antibody produced in rabbit has been used in:
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Biochem/physiol Actions【生化/生理作用】 | Calreticulin facilitates transient interaction with newly synthesized cellular and extracellular proteins for folding and assembling in the endoplasmic reticulum (ER) before its localization to the cytosol or cell surface. Calreticulin acts as a lectin-like chaperone binding oligosaccharide residues of newly synthesized N-linked glycoproteins and misfolded proteins. It is believed to play a critical role in quality control processes during protein synthesis and folding and calcium (Ca2+) homeostasis. Increased expression of calreticulin increases the Ca2+ storage capacity of the ER. It also appears to modulate store-operated Ca2+-influx and to alter Ca2+ transport by the sarcoplasmic/ER Ca2+-adenosine triphosphatase (ATPase) (SERCA). Overexpression of calreticulin results in increased sensitivity of HeLa cells to drug-induced apoptosis. However, increased resistance to apoptosis has been observed in calreticulin-deficient cells. |
Physical form【外形】 | 0.01M 磷酸缓冲盐溶液,pH 7.4,含 15mM 叠氮化钠。 |
产品性质
biological source【生物来源】 | rabbit |
Quality Level【质量水平】 | 200 |
conjugate【偶联物】 | unconjugated |
antibody form【抗体形式】 | IgG fraction of antiserum |
antibody product type | primary antibodies |
clone【克隆】 | polyclonal |
form【形式】 | buffered aqueous solution |
mol wt【分子量】 | antigen 50 kDa |
species reactivity | human, canine |
technique(s) | immunoprecipitation (IP): suitable using whole cell RIPA lysate of the human epitheloid carcinoma HeLa cell line indirect immunofluorescence: 1:100 using 3% paraformaldehyde-fixed, 0.5% Triton X-100 treated, Madin Darby canine kidney MDCK cell line microarray: suitable western blot: 1:2,000 using whole cell RIPA lysate of the human epitheloid carcinoma HeLa cell line |
UniProt accession no.【UniProt登记号】 | P27797 |
shipped in【运输】 | dry ice |
storage temp.【储存温度】 | −20℃ |
Gene Information | human ... CALR(811) |
产品说明
Storage and Stability【储存及稳定性】 | Store at -20 ℃. For continuous use, the product maybe stored at 2-8 ℃ for up to one month. For prolonged storage, freeze in working aliquots at-20 ℃. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded ifnot used within 12 hours. |
Disclaimer【免责声明】 | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
安全信息
Storage Class Code【储存分类代码】 | 12 - Non Combustible Liquids |
WGK | nwg |