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Anti-Chloramphenicol Acetyl Transferase (CAT) antibody produced in rabbit
产品编号: | 3353372 |
规格: | IgG fraction of antiserum, buffered aqueous solution |
包装规格: | 0.5 ML |
产品类别: | 进口试剂 |
品牌: | Sigma-Aldrich |
优惠价: | 立即咨询 |
产品别名
Anti-Chloramphenicol Acetyl Transferase (CAT) antibody produced in rabbit
Cat Antibody
CAT Antibody-Anti-Chloramphenicol Acetyl Transferase (CAT) antibody produced in rabbit
基本信息
MDL number | MFCD01092314 |
General description【一般描述】 | Chloramphenicol Acetyl Transferases (CATs) shows conservation and differences in their amino acid sequences. CAT is encode by cat gene and exists as monomer and later assemble into a trimer. |
Specificity【特异性】 | Anti-Chloramphenicol Acetyl Transferase (CAT) antibody is specific for bacterial CAT and recombinant CAT expressed in transfected eukaryotic cells (a predominant band of approx. 26 kD). |
Immunogen【免疫原】 | bacterial chloramphenicol acetyl transferase (CAT). |
Application【应用】 | Anti-Chloramphenicol Acetyl Transferase (CAT) has been used in
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Biochem/physiol Actions【生化/生理作用】 | Bacterial chloramphenicol acetyl transferase (CAT) is an enzyme that catalyzes the inactivation of the antibiotic, chloramphenicol, by acetylation and subsequently confers bacterial resistance to the antibiotic. CAT, being a stable prokaryotic enzyme, is often used as a reporter gene in transfection assays developed for eukaryotic promoters. Quantification of reporter gene expressions, such as that of CAT, can be correlated to the transcriptional functions of the target sequence. Thus, antibodies directed against CAT can be used for the study of gene sequences that are fused to the CAT reporter gene Anti-Chloramphenicol Acetyl Transferase (CAT) antibody is specific for bacterial CAT and recombinant CAT expressed in transfected eukaryotic cells (a predominant band of approx. 26 kD). Staining of CAT by the antibody is inhibited by the bacterial CAT antigen in cells transfected with CAT. |
Physical form【外形】 | 0.01M 磷酸缓冲盐溶液,pH 7.4,含 15mM 叠氮化钠。 |
产品性质
biological source【生物来源】 | rabbit |
Quality Level【质量水平】 | 200 |
conjugate【偶联物】 | unconjugated |
antibody form【抗体形式】 | IgG fraction of antiserum |
antibody product type | primary antibodies |
clone【克隆】 | polyclonal |
form【形式】 | buffered aqueous solution |
mol wt【分子量】 | antigen 26 kDa |
technique(s) | indirect immunofluorescence: 10 μg/mL using eukaryotic cells transfected with a plasmid bearing the CAT gene western blot: 10 μg/mL using eukaryotic cells transfected with a plasmid bearing the CAT gene |
shipped in【运输】 | dry ice |
storage temp.【储存温度】 | −20℃ |
产品说明
Disclaimer【免责声明】 | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
安全信息
Storage Class Code【储存分类代码】 | 12 - Non Combustible Liquids |
WGK | WGK 3 |